Distinct segment-specific functions of calyculin A-sensitive protein phosphatases in the regulation of cAMP-triggered events in ejaculated bull spermatozoa

Molecular Reproduction and Development
Yohei MizunoHiroshi Harayama

Abstract

Livestock spermatozoa possess more tenacious suppressors of cAMP-triggered events-including capacitation-associated changes-than laboratory animal spermatozoa, leading to flagellar hyperactivation. In order to identify the suppressors, we examined effects of an inhibitor of serine/threonine protein phosphatases (calyculin A) on cAMP-triggered changes in the protein phosphorylation state, and subsequent occurrence of hyperactivation and acrosome reaction in ejaculated bull spermatozoa. Ejaculated spermatozoa were incubated in cAMP-supplemented medium, then assessed for motility, acrosome morphology, and phosphorylated protein localization. The addition of calyculin A greatly enhanced cAMP-triggered protein phosphorylation at serine/threonine and tyrosine residues in the connecting piece and induction of flagellar hyperactivation. Most hyperactivated spermatozoa exhibited extremely asymmetrical bends at the middle piece, which produced intensive twisting or figure-eight movements. In the sperm head, however, cAMP-triggered dephosphorylation of serine/threonine-phosphorylated proteins and subsequent acrosome reaction were abolished by the addition of calyculin A. Based on these results, we suggest that calyculin A-sensitive protei...Continue Reading

References

Mar 1, 1976·Journal of Reproduction and Fertility·R H HunterP J Henfrey
Mar 31, 1989·Biochemical and Biophysical Research Communications·H IshiharaD Uemura
Nov 1, 1987·The Journal of Experimental Zoology·S S SuarezM W Ceglia
Nov 1, 1985·The American Journal of Anatomy·G OliphantT S Thomas
Oct 1, 1968·Journal of Dairy Science·G Igboeli, R H Foote
Mar 1, 1969·Journal of Reproduction and Fertility·R Yanagimachi
Jul 23, 1998·Biology of Reproduction·N L Cross
Aug 31, 2001·Human Reproduction·M LuconiE Baldi
Dec 25, 2003·Molecular Reproduction and Development·R H F Hunter, H Rodriguez-Martinez
Feb 10, 2004·Biology of Reproduction·Becky Marquez, Susan S Suarez
Jul 29, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·Harald Schmidt, Günter Kamp
Mar 8, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·I M AparicioL J Garcia-Marin
Dec 13, 2006·Molecular Reproduction and Development·I M AparicioL J Garcia-Marin
Jul 26, 2008·Human Reproduction Update·Susan S Suarez
Aug 30, 2008·Biology of Reproduction·Natsuko KawanoManabu Yoshida
Feb 27, 2010·Reproduction : the Official Journal of the Society for the Study of Fertility·Tatsuya SuzukiMitsuaki Suzuki
May 6, 2010·Reproduction : the Official Journal of the Society for the Study of Fertility·T RotmanH Breitbart
Jul 6, 2010·Journal of Proteomics·Arlindo A MouraGary J Killian
Aug 10, 2010·Journal of Proteomics·Clémence BelleannéeFrançoise Dacheux
Oct 13, 2010·The Journal of Biological Chemistry·Maya FinkelsteinHaim Breitbart
Mar 11, 2011·Biology of Reproduction·Haixin Chang, Susan S Suarez
Apr 15, 2011·Biology of Reproduction·R John Aitken
May 5, 2011·Asian Journal of Andrology·Pablo E ViscontiAlberto Darszon
Oct 4, 2011·Reproduction : the Official Journal of the Society for the Study of Fertility·Tamara Leahy, Bart M Gadella
Oct 25, 2011·Annual Review of Physiology·Polina V LishkoDavid E Clapham
Dec 20, 2011·Developmental Biology·Sarit Bar-Sheshet ItachHaim Breitbart
Mar 20, 2012·Cell and Tissue Research·Janetti SignorelliPatricio Morales

❮ Previous
Next ❯

Citations

Nov 25, 2016·Molecular Reproduction and Development·Mariko TsukamotoHiroshi Iida
Jun 29, 2018·The Journal of Reproduction and Development·Ayano YamadaHiroshi Harayama
Nov 1, 2018·Reproductive Medicine and Biology·Hiroshi Harayama

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.