Distinct sets of tethering complexes, SNARE complexes, and Rab GTPases mediate membrane fusion at the vacuole in Arabidopsis

Proceedings of the National Academy of Sciences of the United States of America
Kodai TakemotoTakashi Ueda

Abstract

Membrane trafficking plays pivotal roles in various cellular activities and higher-order functions of eukaryotes and requires tethering factors to mediate contact between transport intermediates and target membranes. Two evolutionarily conserved tethering complexes, homotypic fusion and protein sorting (HOPS) and class C core vacuole/endosome tethering (CORVET), are known to act in endosomal/vacuolar transport in yeast and animals. Both complexes share a core subcomplex consisting of Vps11, Vps18, Vps16, and Vps33, and in addition to this core, HOPS contains Vps39 and Vps41, whereas CORVET contains Vps3 and Vps8. HOPS and CORVET subunits are also conserved in the model plant Arabidopsis. However, vacuolar trafficking in plants occurs through multiple unique transport pathways, and how these conserved tethering complexes mediate endosomal/vacuolar transport in plants has remained elusive. In this study, we investigated the functions of VPS18, VPS3, and VPS39, which are core complex, CORVET-specific, and HOPS-specific subunits, respectively. Impairment of these tethering proteins resulted in embryonic lethality, distinctly altering vacuolar morphology and perturbing transport of a vacuolar membrane protein. CORVET interacted with...Continue Reading

References

Mar 31, 1999·The Plant Journal : for Cell and Molecular Biology·S ManoM Nishimura
Aug 16, 2000·Proceedings of the National Academy of Sciences of the United States of America·D F SealsA Price
Jul 13, 2001·The Journal of Cell Biology·S CaplanJ S Bonifacino
May 7, 2002·Trends in Plant Science·Mansour KarimiAnn Depicker
Feb 18, 2003·Molecular Biology of the Cell·Enrique RojoNatasha V Raikhel
Nov 18, 2004·The Plant Journal : for Cell and Molecular Biology·Takashi UedaAkihiko Nakano
Mar 4, 2005·The Plant Journal : for Cell and Molecular Biology·Judith CraftIan Moore
Jan 31, 2006·The Plant Journal : for Cell and Molecular Biology·Ian MooreSmita Kurup
Mar 15, 2006·The Plant Cell·Rebecca SchwabDetlef Weigel
May 1, 2008·The Plant Journal : for Cell and Molecular Biology·Masatoshi YamaguchiTaku Demura
Jul 12, 2008·Nature Structural & Molecular Biology·William Wickner, Randy Schekman
Mar 25, 2009·The Plant Journal : for Cell and Molecular Biology·Niko GeldnerJoanne Chory
Jul 7, 2009·Current Opinion in Cell Biology·Daniel P NickersonAlexey J Merz
Nov 4, 2009·Plant & Cell Physiology·Mitsuru NiihamaMiyo Terao Morita
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Melanie KrebsKarin Schumacher
Aug 28, 2010·Current Biology : CB·Mirjana NordmannChristian Ungermann
Jun 15, 2011·Nature Cell Biology·Kazuo EbineTakashi Ueda
Sep 14, 2011·The Plant Journal : for Cell and Molecular Biology·Emi ItoAkihiko Nakano
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
May 7, 2013·Journal of Cell Science·Henning J kleine Balderhaar, Christian Ungermann
Jan 1, 2014·PloS One·Athanasios LampropoulosJoachim Forner
Feb 22, 2014·Plant & Cell Physiology·Masayuki FujiwaraYoichiro Fukao
Jun 3, 2014·Current Biology : CB·Kazuo EbineTakashi Ueda
Oct 14, 2014·Frontiers in Plant Science·Chunhua ZhangNatasha V Raikhel
Mar 10, 2015·Cellular Logistics·Jens LachmannChristian Ungermann
May 18, 2016·Proceedings of the National Academy of Sciences of the United States of America·Lihong HaoLi-Jia Qu
Jun 1, 2016·Frontiers in Cell and Developmental Biology·Nemanja Vukašinović, Viktor Žárský

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Citations

Jun 24, 2019·The New Phytologist·Liang ZhangJinxing Lin
Oct 16, 2019·Plant Signaling & Behavior·Michel Ruiz RosqueteGeorgia Drakakaki
Oct 2, 2019·Proceedings of the National Academy of Sciences of the United States of America·Cecilia Rodriguez-FurlanGlenn R Hicks
Jan 23, 2020·Journal of Cell Science·Daniela SparvoliAaron P Turkewitz
Apr 9, 2020·Cells·Katarzyna SieńkoShino Goto-Yamada
Aug 15, 2018·Proceedings of the National Academy of Sciences of the United States of America·Carla BrilladaMarcela Rojas-Pierce
Dec 12, 2019·Wellcome Open Research·Cordula Boehm, Mark C Field
Jun 18, 2020·International Journal of Molecular Sciences·Yiran CaoDabing Zhang
Jun 18, 2020·Frontiers in Plant Science·Cheuk-Ling WunXiaohong Zhuang
Sep 25, 2020·Proceedings of the National Academy of Sciences of the United States of America·Masaru FujimotoTakashi Ueda
Mar 4, 2020·Plant Molecular Biology·Prakash B AdhikariRyushiro D Kasahara
May 28, 2020·Frontiers in Plant Science·Sabrina Kaiser, David Scheuring
Apr 24, 2020·Proceedings of the National Academy of Sciences of the United States of America·María Otilia DelgadilloEnrique Rojo
Sep 5, 2018·Journal of Cell Science·Xingyun QiDaniël Van Damme
Dec 19, 2019·Frontiers in Plant Science·Shino Goto-YamadaKenji Yamada
Oct 30, 2020·Evolutionary Bioinformatics Online·Xiaoyan GuKeith Lindsey
Jan 29, 2021·Life·Olivia L McGovernVern B Carruthers
May 15, 2020·Trends in Plant Science·Yong CuiLiwen Jiang
Apr 2, 2021·Plant Physiology·Zhiqi LiuLiwen Jiang
Jul 13, 2021·Frontiers in Plant Science·Heike WolffMartin Hülskamp
Jun 5, 2021·Plant & Cell Physiology·Hiroyuki IshidaKlaas J van Wijk
Sep 23, 2021·The Plant Cell·Fernando AnientoEugenia Russinova
Sep 30, 2021·The Plant Cell·Leia ColinYvon Jaillais
Oct 12, 2021·The Plant Cell·Byung-Ho KangBethany Karlin Zolman

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Methods Mentioned

BETA
GTPase
GTPases
two-hybrid
transgenic
Coimmunoprecipitation
immunoprecipitation
nucleotide exchange
genotyping
confocal microscopy
PCR

Software Mentioned

HOPS
Image J
Fiji
CORVET
GreenGate
Leica Application Suite Advanced Fluorescence
Proteome Discoverer
ImageJ
OriginPro
WMD3

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