Distinct temporal genetic signatures of neurogenic and gliogenic cues in cortical stem cell cultures

Journal of Neurobiology
Claire SauvageotCharles D Stiles

Abstract

Cortical progenitor cells from rat embryos give rise to neurons or glia following exposure to platelet derived growth factor (PDGF) or ciliary neurotrophic factor (CNTF), respectively. Both growth factors impart their developmental cues quickly through a transcription-dependent mechanism. Do the alternate developmental responses to PDGF and CNTF reflect induction of qualitatively distinct genes? Alternatively, do the same genes respond to each growth factor, but with quantitatively distinct kinetics? Using differential library screening and custom cDNA microarrays we show that a common set of genes responds to either growth factor. However, quantitative differences in the onset and duration of gene induction equate to the expression of factor-specific gene signatures. Multitissue cluster analysis also reveals tissue-specific gene signatures that may play important roles in the developing brain.

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Citations

Dec 22, 2010·Proceedings of the National Academy of Sciences of the United States of America·Katherine A JanewayConstantine A Stratakis
Dec 18, 2013·Human Molecular Genetics·Luis Jaime Castro-VegaAnne-Paule Gimenez-Roqueplo
May 21, 2010·Human Molecular Genetics·Nelly BurnichonAnne-Paule Gimenez-Roqueplo
Jul 26, 2011·Human Molecular Genetics·Nelly BurnichonAnne-Paule Gimenez-Roqueplo
Sep 27, 2012·Human Molecular Genetics·Jenny WelanderOliver Gimm
Apr 19, 2008·Journal of Neurochemistry·Anayansi Molina-Hernández, Iván Velasco
Jun 30, 2006·Journal of Neurochemistry·Yoko IshiiMasakiyo Sasahara
Feb 7, 2013·Foot & Ankle International·Daniel S McNeilMark A Glazebrook

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