Distinct, tissue-specific regulation of vitamin D receptor in the intestine, kidney, and skin by dietary calcium and vitamin D

Endocrinology
R ZinebR R Marthe

Abstract

We studied the effects of vitamin D deficiency and its correction by vitamin D or calcium-lactose supplementation on vitamin D receptor (VDR) expression in skin keratinocytes, kidney, and duodenum of adult rats. VDR messenger RNA (mRNA) was assayed by Northern blot, and VDR protein was determined immunocytochemically. In addition, four subpopulations of keratinocytes were isolated, characterized for their stages of differentiation, and analyzed for VDR expression. Vitamin D deficiency decreased VDR mRNA in all three tissues. Treatment with vitamin D or calcium-lactose reestablished the VDR mRNA content of the epidermis, but not that of the kidneys, and only the calcium-lactose diet increased duodenal VDR mRNA. The regulation of VDR mRNA in the epidermis was independent of cell differentiation, whereas VDR protein varied with differentiation. The VDR-positive cells in the control rats were at early and advanced states of differentiation. The expression of VDR was decreased by vitamin D deficiency and returned to control values after vitamin D or calcium supplementation. Vitamin D treatment, but not calcium, induced VDR expression in the normally immature population. Vitamin D and calcium, therefore, have distinct, tissue-specifi...Continue Reading

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Citations

Nov 14, 2008·Journal of the American Society of Nephrology : JASN·Joanne MarksRobert J Unwin
Jul 17, 2013·Best Practice & Research. Clinical Endocrinology & Metabolism·Chia-Ling Tu, Daniel D Bikle
Apr 28, 2012·Experimental and Molecular Pathology·Gaurav K GuptaDevendra K Agrawal
Oct 16, 2007·Journal of Morphology·Katharina RinerAnnette Liesegang
Mar 27, 2010·General and Comparative Endocrinology·Pia Bagamasbad, Robert J Denver
Jan 6, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Jun QinSophia Y Tsai
May 10, 2007·Cells, Tissues, Organs·Alois BoosAnnette Liesegang
Apr 3, 2010·Journal of the American Society of Nephrology : JASN·János Peti-Peterdi, Raymond C Harris
Jan 9, 2013·European Journal of Clinical Investigation·Chiao-Lin ChuangShou-Dong Lee
Dec 28, 2012·Journal of Lipid Research·Cecilia I CasaliMaría C Fernández Tome
Nov 27, 2019·Organic & Biomolecular Chemistry·Kouta IbeSentaro Okamoto

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