Distinct TLR- and NLR-mediated transcriptional responses to an intracellular pathogen

PLoS Pathogens
Jess H LeberDaniel A Portnoy

Abstract

How the innate immune system tailors specific responses to diverse microbial infections is not well understood. Cells use a limited number of host receptors and signaling pathways to both discriminate among extracellular and intracellular microbes, and also to generate responses commensurate to each threat. Here, we have addressed these questions by using DNA microarrays to monitor the macrophage transcriptional response to the intracellular bacterial pathogen Listeria monocytogenes. By utilizing combinations of host and bacterial mutants, we have defined the host transcriptional responses to vacuolar and cytosolic bacteria. These compartment-specific host responses induced significantly different sets of target genes, despite activating similar transcription factors. Vacuolar signaling was entirely MyD88-dependent, and induced the transcription of pro-inflammatory cytokines. The IRF3-dependent cytosolic response induced a distinct set of target genes, including IFNbeta. Many of these cytosolic response genes were induced by secreted cytokines, so we further identified those host genes induced independent of secondary signaling. The host response to cytosolic bacteria was reconstituted by the cytosolic delivery of L. monocytoge...Continue Reading

References

Apr 1, 1988·The Journal of Experimental Medicine·D A PortnoyD J Hinrichs
Jan 1, 1997·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·B Lorber
Jun 29, 1999·Cold Spring Harbor Symposia on Quantitative Biology·T ManiatisM G Wathelet
Oct 12, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·G L Blatch, M Lässle
Mar 17, 2000·Immunological Reviews·R Medzhitov, C Janeway
Nov 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·S SanjabiS T Smale
Apr 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·V G TusherG Chu
Sep 20, 2001·Genes & Development·N Silverman, T Maniatis
Mar 22, 2002·Nature Reviews. Immunology·R Medzhitov
Oct 3, 2002·Proceedings of the National Academy of Sciences of the United States of America·Mary O'RiordanDaniel A Portnoy
Jan 16, 2003·The Journal of Biological Chemistry·Stephen E GirardinPhilippe J Sansonetti
Jun 7, 2003·The Journal of Infectious Diseases·Alan Aderem
Aug 20, 2003·Cellular Microbiology·Mathias ChamaillardDana J Philpott
Oct 15, 2003·Proceedings of the National Academy of Sciences of the United States of America·Sarah A StanleyJeffery S Cox
Dec 3, 2003·Trends in Immunology·Stephen E GirardinPhilippe J Sansonetti
Jul 23, 2004·Proceedings of the National Academy of Sciences of the United States of America·Ramona L McCaffreyDaniel A Portnoy
Aug 11, 2004·The Journal of Experimental Medicine·Victoria AuerbuchDaniel A Portnoy
Aug 11, 2004·The Journal of Experimental Medicine·Javier A CarreroEmil R Unanue
Aug 11, 2004·The Journal of Experimental Medicine·Ryan M O'ConnellGenhong Cheng
Oct 19, 2004·Nature Immunology·Jérôme VialaRichard L Ferrero
Dec 9, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Silvia StockingerThomas Decker
Jan 22, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ryan M O'ConnellGenhong Cheng
Feb 5, 2005·Science·Koichi S KobayashiRichard A Flavell
May 17, 2005·Immunity·Lynda M Stuart, R Alan B Ezekowitz
Jun 15, 2005·Annual Review of Biochemistry· InoharaGabriel Nuñez
Jul 6, 2005·Nature·Myrna Watanabe
Sep 17, 2005·Genes & Development·Shomyseh SanjabiStephen T Smale
Jan 18, 2006·Cell Death and Differentiation·T Kawai, S Akira
Jan 18, 2006·Immunity·Daniel B Stetson, Ruslan Medzhitov
Feb 24, 2006·Nature Reviews. Immunology·Warren StroberTomohiro Watanabe
Feb 25, 2006·Cell·Shizuo AkiraOsamu Takeuchi
Mar 23, 2006·Annual Review of Immunology·Bruce BeutlerKasper Hoebe
Mar 23, 2006·BMC Genomics·Ricardo A Verdugo, Juan F Medrano

❮ Previous
Next ❯

Citations

May 9, 2013·Clinical & Developmental Immunology·Ashley M Sherrid, Tobias R Kollmann
Jul 20, 2012·Clinical & Developmental Immunology·Sabrina DominiciGiorgio Brandi
Jul 18, 2008·Proceedings of the National Academy of Sciences of the United States of America·Gregory T CrimminsDaniel A Portnoy
Nov 15, 2008·PLoS Pathogens·György FejerMarina A Freudenberg
Apr 16, 2009·Clinical Microbiology Reviews·Trine H Mogensen
May 19, 2009·Proceedings of the National Academy of Sciences of the United States of America·Garabet YeretssianMaya Saleh
Jul 8, 2009·The Journal of Experimental Medicine·François CoulombeMarcel A Behr
Aug 5, 2009·The Journal of Experimental Medicine·Sarah M McWhirterRussell E Vance
Dec 10, 2009·PLoS Pathogens·Victoria AuerbuchRalph R Isberg
Feb 4, 2010·The Journal of Experimental Medicine·Manira RayamajhiLaurel L Lenz
Oct 6, 2010·Genome Biology·Sarah M Fortune, Eric J Rubin
Dec 3, 2010·The Journal of Biological Chemistry·Eugenio Carrasco-MarínCarmen Alvarez-Dominguez
Dec 3, 2010·PLoS Pathogens·Tamas DolowschiakMathias W Hornef
Feb 19, 2011·American Journal of Respiratory Cell and Molecular Biology·Dane Parker, Alice Prince
Jun 29, 2011·Proceedings of the National Academy of Sciences of the United States of America·Daniel E ZakAlan Aderem
Sep 22, 2011·BMC Systems Biology·Luigi BuonaguroFrancesco M Marincola
Feb 9, 2012·International Journal of Molecular Sciences·Benjamin IzarTorsten Hain
Feb 9, 2012·Proceedings of the National Academy of Sciences of the United States of America·Giovanna ClavarinoPhilippe Pierre
Feb 11, 2012·Critical Reviews in Immunology·Uma Nagarajan
Jan 29, 2011·Clinical & Developmental Immunology·Hiroyuki SaigaKiyoshi Takeda

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE8104

Methods Mentioned

BETA
dissection
nuclear translocation
transfection
PCR
Infrared Imaging

Software Mentioned

Significance Analysis of Microarrays ( SAM )
Excel
SAM
Acuity
GenePix Pro
SpotReader
MxPro

Related Concepts

Related Feeds

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.