Distinct transgenic effects of poplar TDIF genes on vascular development in Arabidopsis

Plant Cell Reports
Xin LiJiehua Wang

Abstract

Poplar CLE genes encoding TDIF motifs differentially regulate vascular cambial cell division and woody tissue organization in transgenic Arabidopsis. In Arabidopsis, CLE41 and CLE44 genes encode the tracheary element differentiation inhibitory factor (TDIF) peptide, which functions as a non-cell autonomous signal to regulate vascular development, and overexpression of AtCLE41/CLE44 generate similar phenotypic defects. In poplar, there are six CLE genes (PtTDIF1-4 and PtTDIF-like1-2) encoding two TDIF peptides (TDIF and TDIF-like peptide), which exhibit nearly same activities when exogenously applied to Arabidopsis seedlings. In this work, for each TDIF peptide, we chose two poplar CLE genes (PtTDIF2 and 3 for TDIF, and PtTDIF-like1-2 for TDIF-like peptide) to compare their in vivo effects in transgenic Arabidopsis. Our results showed that transgenic Arabidopsis lines overexpressing each individual PtTDIF gene exhibited dramatically distinct phenotypes associated with vascular development, demonstrating that TDIF motif is not the only functional determinant after genetic transformation. Moreover, we revealed that overexpressed poplar TDIFs enhanced the proliferation of (pro)cambial cells only in hypocotyls, but not in infloresce...Continue Reading

References

Mar 9, 1999·The Plant Journal : for Cell and Molecular Biology·S J Clough, A F Bent
Nov 24, 1999·The Plant Cell·F M CarlandB Keith
Jul 18, 2001·Plant Physiology·J M Cock, S McCormick
Feb 25, 2003·Trends in Plant Science·Addie Nina Olsen, Karen Skriver
May 4, 2004·Nature Reviews. Molecular Cell Biology·Hiroo Fukuda
Aug 12, 2006·Science·Tatsuhiko KondoYouji Sakagami
Nov 30, 2006·Current Opinion in Plant Biology·Martijn FiersChun-Ming Liu
Oct 2, 2007·Current Opinion in Plant Biology·Hiroo FukudaShinichiro Sawa
Nov 24, 2007·Cellular and Molecular Life Sciences : CMLS·J H JunJ C Fletcher
Sep 25, 2008·Proceedings of the National Academy of Sciences of the United States of America·Yuki HirakawaHiroo Fukuda
Nov 18, 2008·Proceedings of the National Academy of Sciences of the United States of America·Ryan WhitfordPierre Hilson
Dec 18, 2009·Protoplasma·Guodong Wang, Martijn Fiers
Jan 16, 2010·Journal of Integrative Plant Biology·Yuki HirakawaHiroo Fukuda
Apr 21, 2010·Plant Signaling & Behavior·J Peter Etchells, Simon R Turner
Nov 6, 2010·Plant Molecular Biology·Jun NiSteven E Clark
Jun 23, 2011·Plant & Cell Physiology·Hideyuki YaginumaHiroo Fukuda
Apr 14, 2015·Current Biology : CB·J Peter EtchellsSimon R Turner
Aug 9, 2016·Nature Communications·Junko MoritaOsamu Nureki

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
PCR
light microscopy
glycosylation

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.