Distinct type of transmission barrier revealed by study of multiple prion determinants of Rnq1.

PLoS Genetics
Michele L KadnarI L Derkatch

Abstract

Prions are self-propagating protein conformations. Transmission of the prion state between non-identical proteins, e.g. between homologous proteins from different species, is frequently inefficient. Transmission barriers are attributed to sequence differences in prion proteins, but their underlying mechanisms are not clear. Here we use a yeast Rnq1/[PIN(+)]-based experimental system to explore the nature of transmission barriers. [PIN(+)], the prion form of Rnq1, is common in wild and laboratory yeast strains, where it facilitates the appearance of other prions. Rnq1's prion domain carries four discrete QN-rich regions. We start by showing that Rnq1 encompasses multiple prion determinants that can independently drive amyloid formation in vitro and transmit the [PIN(+)] prion state in vivo. Subsequent analysis of [PIN(+)] transmission between Rnq1 fragments with different sets of prion determinants established that (i) one common QN-rich region is required and usually sufficient for the transmission; (ii) despite identical sequences of the common QNs, such transmissions are impeded by barriers of different strength. Existence of transmission barriers in the absence of amino acid mismatches in transmitting regions indicates that ...Continue Reading

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Citations

Sep 6, 2012·Proceedings of the National Academy of Sciences of the United States of America·Amy C KellyReed B Wickner
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Methods Mentioned

BETA
Assay
NMR
protein

Software Mentioned

Gatan Digital Micrograph
Improvision OpenLab

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