PMID: 2110471Mar 13, 1990Paper

Distinction between the two basic mechanisms of cation transport in the cardiac Na(+)-Ca2+ exchange system

Biochemistry
Daniel Khananshvili

Abstract

In order to distinguish between the Ping-Pong and sequential mechanisms of cation transport in the cardiac Na(+)-Ca2+ exchange system, the initial rates of the Nai-dependent 45Ca uptake (t = 1 s) were measured in reconstituted proteoliposomes, loaded with a Ca chelator. Under "zero-trans" conditions ([Na]o = [Ca]i = 0) at a fixed [Na]i = 10-160 mM with varying [45Ca]o = 2.5-122 microM for each [Na]i, the Km and Vmax values increased from 7.7 to 33.5 microM and from 2.3 to 9.0 nmol.mg-1.s-1, respectively. The Vmax/Km values show a +/- 2-10% deviation from the average value of 0.274 nmol.mg-1.s-1.microM-1 over the whole range of [Na]i. These deviations are within the standard error of Vmax (+/- 3-7%), Km (+/- 11-17%), and Vmax/Km (+/- 11-19%). This suggests that, under conditions in which Vmax and Km are [Na]i dependent and vary 4-5-fold, the Vmax/Km values are constant within the experimental error. In the presence of K(+)-valinomycin the Vmax/Km values are 0.85 +/- 0.17 and 1.08 +/- 0.18 nmol.mg-1.s-1.microM-1 at [Na]i = 20 and 160 mM, respectively, suggesting that under conditions of "short circuit" of the membrane potential the Vmax/Km values still exhibit the [Na]i independence. At a very low fixed [45Ca]o = 1.1 microM with ...Continue Reading

References

Jan 1, 1987·The Journal of Membrane Biology·P Läuger
Dec 22, 1988·Biochimica Et Biophysica Acta·M CondrescuR DiPolo
Jan 1, 1985·Annual Review of Physiology·K D Philipson
May 1, 1973·Analytical Biochemistry·P W Holloway
Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·C C HaleJ P Reeves
Mar 23, 1983·Biochimica Et Biophysica Acta·R F Ledvora, C Hegyvary
Nov 1, 1981·Quarterly Reviews of Biophysics·T L Hill, E Eisenberg
Oct 31, 1958·The Journal of Physiology·H C LUTTGAU, R NIEDERGERKE

❮ Previous
Next ❯

Citations

May 29, 1998·Journal of Clinical Pharmacology·P R YasharW H Frishman
Nov 28, 2013·Pflügers Archiv : European journal of physiology·Daniel Khananshvili
Jan 1, 1989·Progress in Biophysics and Molecular Biology·P P Schnetkamp
Jan 1, 1991·Annals of the New York Academy of Sciences·J M Li, J Kimura
Jan 1, 1991·Annals of the New York Academy of Sciences·D W HilgemannG A Nagel
Jan 1, 1991·Annals of the New York Academy of Sciences·D C GadsbyM Nakao
Jan 1, 1991·Annals of the New York Academy of Sciences·J T DurkinJ P Reeves
Jun 9, 2000·Annual Review of Physiology·K D Philipson, D A Nicoll
Oct 12, 2000·Biochemical and Biophysical Research Communications·R HillerD Khananshvili
Aug 1, 1992·Current Opinion in Cell Biology·K D Philipson, D A Nicoll
Feb 16, 2016·Scientific Reports·Moshe GiladiDaniel Khananshvili
Feb 24, 2016·Frontiers in Physiology·Moshe GiladiDaniel Khananshvili
Dec 4, 2014·Proceedings of the National Academy of Sciences of the United States of America·Fabrizio MarinelliJosé D Faraldo-Gómez
Nov 29, 2002·The Journal of Physiology·Masamitsu HinataJunko Kimura
Apr 15, 1996·Annals of the New York Academy of Sciences·D W Hilgemann
Apr 15, 1996·Annals of the New York Academy of Sciences·M P BlausteinR S Rogowski
Feb 1, 1992·Archives of Biochemistry and Biophysics·J P Reeves
Oct 27, 2004·Biochemical and Biophysical Research Communications·Chagit ShpakDaniel Khananshvili
Jun 20, 2006·Biochemical and Biophysical Research Communications·Boyman LironDaniel Khananshvili
Oct 18, 2005·Biochemical and Biophysical Research Communications·Liron BoymanDaniel Khananshvili
Aug 2, 2003·Biochemical and Biophysical Research Communications·Chagit ShpakDaniel Khananshvili
Nov 24, 2016·International Journal of Molecular Sciences·Moshe GiladiDaniel Khananshvili
Jun 3, 2017·The Journal of Biological Chemistry·Moshe GiladiDaniel Khananshvili
Dec 15, 2017·The Journal of General Physiology·Irina ShlosmanJoseph A Mindell
May 23, 2001·Circulation Research·M Shigekawa, T Iwamoto
Jul 7, 1995·The Journal of Biological Chemistry·D KhananshviliD Baazov
Apr 22, 2017·Scientific Reports·Moshe GiladiDaniel Khananshvili
Aug 5, 1998·The American Journal of Physiology·J I Kourie
Jan 1, 1991·Annals of the New York Academy of Sciences·D Khananshvili
Jul 3, 1999·Physiological Reviews·M P Blaustein, W J Lederer

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.