PMID: 6111455Feb 1, 1981Paper

Distinction between two subpopulations of beta 1-adrenergic receptors in human adipose cells

European Journal of Biochemistry
B JacobssonA D Strosberg

Abstract

The beta-adrenergic receptors in human adipose membranes were identified by the specific and saturable binding of the beta-adrenergic antagonist (--)-[3H]dihydroalprenolol. The total number of sites in control membranes was 0.32 +/- 0.03 pmol/mg protein and the equilibrium dissociation constant for binding (Kd) was 2.6 nM and 2.5 nM as determined by Scatchard analysis of experiments on equilibrium binding and kinetics, respectively. The beta 1-adrenergic nature of the receptors was derived from the order of potencies of beta-adrenergic agonists (isoproterenol greater than norepinephrine greater than epinephrine) to complete with (--)-[3H]dihydroalprenolol for binding. Studies of saturation binding, kinetics and competition binding revealed the presence of a single class of beta 1-adrenergic receptors. Prolonged incubation of human adipose cells in the presence of (--)-norepinephrine decreases the lipolytic response to beta-adrenergic agonists, and reduces by 50% the concentration of beta-adrenergic receptors. The Kd values for (--)-[3H]dihydroalprenolol and the beta-adrenergic agonists remain unchanged. Catecholamines also produce a rapid conformational change of approximatively 50% of the receptors in control membranes as reve...Continue Reading

References

Feb 28, 1979·Biochemical and Biophysical Research Communications·S BottariA D Strosberg
Jan 30, 1976·European Journal of Clinical Investigation·U SmithL Sjörstrõm
Oct 1, 1977·European Journal of Clinical Investigation·U SmithB Jacobsson
Jan 1, 1978·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·H KatherB Simon

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Citations

Apr 1, 1988·Journal of Endocrinological Investigation·C TantucciV Grassi
Aug 22, 1986·Pharmaceutisch Weekblad. Scientific Edition·A P Ijzerman, H Timmerman
Feb 1, 1991·Journal of Clinical Pharmacy and Therapeutics·M J KendallB A Hughes
Jan 1, 1993·Journal of Neurochemistry·R J Summers, L R McMartin
Jun 15, 1982·European Journal of Biochemistry·G VauquelinA D Strosberg
Jan 1, 1985·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·T B Ng
Mar 1, 1991·Biochemical Pharmacology·L J EmorineD A Strosberg
Oct 14, 1982·Biochimica Et Biophysica Acta·P Björgell, P Belfrage
May 16, 1983·Biochemical and Biophysical Research Communications·P Lönnroth, U Smith

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