Distinctive Archaeal Composition of an Artisanal Crystallizer Pond and Functional Insights Into Salt-Saturated Hypersaline Environment Adaptation

Frontiers in Microbiology
Alvaro M PlominskyNicole Trefault

Abstract

Hypersaline environments represent some of the most challenging settings for life on Earth. Extremely halophilic microorganisms have been selected to colonize and thrive in these extreme environments by virtue of a broad spectrum of adaptations to counter high salinity and osmotic stress. Although there is substantial data on microbial taxonomic diversity in these challenging ecosystems and their primary osmoadaptation mechanisms, less is known about how hypersaline environments shape the genomes of microbial inhabitants at the functional level. In this study, we analyzed the microbial communities in five ponds along the discontinuous salinity gradient from brackish to salt-saturated environments and sequenced the metagenome of the salt (halite) precipitation pond in the artisanal Cáhuil Solar Saltern system. We combined field measurements with spectrophotometric pigment analysis and flow cytometry to characterize the microbial ecology of the pond ecosystems, including primary producers and applied metagenomic sequencing for analysis of archaeal and bacterial taxonomic diversity of the salt crystallizer harvest pond. Comparative metagenomic analysis of the Cáhuil salt crystallizer pond against microbial communities from other s...Continue Reading

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Citations

Mar 17, 2019·Genes·Gherman Uritskiy, Jocelyne DiRuggiero
Nov 22, 2019·Frontiers in Microbiology·Sonia Dávila-RamosRamón Alberto Batista-García
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Susanna GorrasiMassimiliano Fenice

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Datasets Mentioned

BETA
SRX680116
PRJNA388720

Methods Mentioned

BETA
flow cytometry
Light Scattering
PCR
electrophoresis

Software Mentioned

MAFFT
MEGA
Newbler
DIAMOND
Tundra Pie - Chart editor
fastq
Spigot
R
FlowJo
Geneious

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