Distinctive gut microbiota of honey bees assessed using deep sampling from individual worker bees.

PloS One
Nancy A MoranZakee L Sabree

Abstract

Surveys of 16S rDNA sequences from the honey bee, Apis mellifera, have revealed the presence of eight distinctive bacterial phylotypes in intestinal tracts of adult worker bees. Because previous studies have been limited to relatively few sequences from samples pooled from multiple hosts, the extent of variation in this microbiota among individuals within and between colonies and locations has been unclear. We surveyed the gut microbiota of 40 individual workers from two sites, Arizona and Maryland USA, sampling four colonies per site. Universal primers were used to amplify regions of 16S ribosomal RNA genes, and amplicons were sequenced using 454 pyrotag methods, enabling analysis of about 330,000 bacterial reads. Over 99% of these sequences belonged to clusters for which the first blastn hits in GenBank were members of the known bee phylotypes. Four phylotypes, one within Gammaproteobacteria (corresponding to "Candidatus Gilliamella apicola") one within Betaproteobacteria ("Candidatus Snodgrassella alvi"), and two within Lactobacillus, were present in every bee, though their frequencies varied. The same typical bacterial phylotypes were present in all colonies and at both sites. Community profiles differed significantly among...Continue Reading

References

Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Jan 9, 2004·Applied and Environmental Microbiology·Nichole A BroderickJo Handelsman
Apr 20, 2004·Journal of Bacteriology·Silvia G AcinasMartin F Polz
Aug 2, 2006·Proceedings of the National Academy of Sciences of the United States of America·Mitchell L SoginGerhard J Herndl
Mar 27, 2007·FEMS Microbiology Ecology·Dirk BabendreierFranco Widmer
Apr 3, 2007·Applied and Environmental Microbiology·Vanessa Corby-HarrisDaniel E L Promislow
Jun 26, 2007·Applied and Environmental Microbiology·Qiong WangJames R Cole
Sep 8, 2007·Science·Diana L Cox-FosterW Ian Lipkin
Aug 4, 2009·PloS One·Dennis VanengelsdorpJeffery S Pettis
Jan 22, 2010·The ISME Journal·Anna EngelbrektsonPhilip Hugenholtz
Sep 21, 2010·Applied and Environmental Microbiology·Elena CrottiDaniele Daffonchio
Dec 24, 2010·Molecular Ecology·Vincent G MartinsonNancy A Moran
May 11, 2011·Microbial Ecology·Hauke Koch, Paul Schmid-Hempel
Jun 17, 2011·Nature·Andrew L KauJeffrey I Gordon
Feb 7, 2012·Applied and Environmental Microbiology·Vincent G MartinsonNancy A Moran

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Citations

Apr 18, 2014·PloS One·Vanessa Corby-HarrisKirk E Anderson
Sep 10, 2014·Nature Reviews. Microbiology·Gemma Langridge
Jul 24, 2014·Proceedings of the National Academy of Sciences of the United States of America·Waldan K KwongNancy A Moran
Jun 20, 2012·Proceedings of the National Academy of Sciences of the United States of America·Philipp EngelNancy A Moran
Apr 25, 2013·PloS One·Ryan Thomas JonesNoah Fierer
Sep 13, 2013·BMC Research Notes·Matan ShelomiChih-Horng Kuo
May 31, 2013·The ISME Journal·Adam C-N WongAngela E Douglas
Jul 13, 2013·Systematic and Applied Microbiology·Akihito Endo, Seppo Salminen
Mar 1, 2016·Frontiers in Microbiology·Jacquelynn Benjamino, Joerg Graf
Nov 6, 2015·International Journal of Systematic and Evolutionary Microbiology·Lucie KešnerováPhilipp Engel
Oct 8, 2014·BMC Microbiology·Amber R PaulsonSteve J Perlman
May 2, 2014·Environmental Entomology·Charles J Mason, Kenneth F Raffa
Jun 4, 2013·New Biotechnology·Elena CrottiAnnalisa Balloi
Jan 23, 2016·Journal of Immunology Research·Laura M Brutscher, Michelle L Flenniken
Aug 25, 2015·International Journal of Systematic and Evolutionary Microbiology·Van-An HoangDeok-Chun Yang
Jan 26, 2013·Molecular Ecology·Hauke KochPaul Schmid-Hempel
Jun 7, 2014·Environmental Microbiology·Fredrick J LeeIrene L G Newton
Aug 21, 2013·Molecular Ecology·Allison K Hansen, Nancy A Moran
Jan 9, 2016·International Journal of Systematic and Evolutionary Microbiology·Waldan K Kwong, Nancy A Moran
May 27, 2015·Gut Microbes·Waldan K Kwong, Nancy A Moran
Apr 23, 2013·International Journal of Systematic and Evolutionary Microbiology·Philipp EngelNancy A Moran
Aug 15, 2015·Current Opinion in Insect Science·Laura M BrutscherMichelle L Flenniken
Jul 4, 2015·Current Opinion in Insect Science·Nancy A Moran
May 23, 2015·Environmental Microbiology Reports·Lindsay K NewboldChristopher J van der Gast
Aug 24, 2013·Journal of Microbiological Methods·Alissa S HanshewCameron R Currie
May 23, 2013·FEMS Microbiology Reviews·Philipp Engel, Nancy A Moran
Apr 17, 2015·BMC Genomics·Kirsten M EllegaardAlejandra Vásquez
Apr 9, 2015·Frontiers in Genetics·Cansu Ö TozkarJay D Evans

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Datasets Mentioned

BETA
SRA046735
JQ582009-JQ582011

Methods Mentioned

BETA
dissection
454 Titanium sequencing
PCR

Software Mentioned

Greengenes Bellerpheron
DNAsp package
Geneious
MEGA
blastn
MUSCLE
RDP Classifier
Perl
PyroTagger
PCORD

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