Distinctive Properties of Dark Reversion Kinetics between Two Red/Green-Type Cyanobacteriochromes and their Application in the Photoregulation of cAMP Synthesis

Photochemistry and Photobiology
Keiji FushimiRei Narikawa

Abstract

Cyanobacteriochromes (CBCRs) are photoreceptors that bind to a linear tetrapyrrole within a conserved cGMP-phosphodiesterase/adenylate cyclase/FhlA (GAF) domain and exhibit reversible photoconversion. Red/green-type CBCR GAF domains that photoconvert between red- (Pr) and green-absorbing (Pg) forms occur widely in various cyanobacteria. A putative phototaxis regulator, AnPixJ, contains multiple red/green-type CBCR GAF domains. We previously reported that AnPixJ's second domain (AnPixJg2) but not its fourth domain (AnPixJg4) shows red/green reversible photoconversion. Herein, we found that AnPixJg4 showed Pr-to-Pg photoconversion and rapid Pg-to-Pr dark reversion, whereas AnPixJg2 showed a barely detectable dark reversion. Site-directed mutagenesis revealed the involvement of six residues in Pg stability. Replacement at the Leu294/Ile660 positions of AnPixJg2/AnPixJg4 showed the highest influence on dark reversion kinetics. AnPixJg2_DR6, wherein the six residues of AnPixJg2 were entirely replaced with those of AnPixJg4, showed a 300-fold faster dark reversion than that of the wild type. We constructed chimeric proteins by fusing the GAF domains with adenylate cyclase catalytic regions, such as AnPixJg2-AC, AnPixJg4-AC and AnPixJ...Continue Reading

References

Jul 1, 1986·Analytical Biochemistry·T R Berkelman, J C Lagarias
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·J SchultzC P Ponting
Jul 12, 2002·The EMBO Journal·Tobias KanacherJoachim E Schultz
Dec 1, 1959·Proceedings of the National Academy of Sciences of the United States of America·W L ButlerS B Hendricks
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·W RüdigerS Schneider
May 4, 2006·Annual Review of Plant Biology·Nathan C RockwellJ Clark Lagarias
Jun 24, 2008·Journal of Molecular Biology·Rei NarikawaMasahiko Ikeuchi
Jul 16, 2008·Proceedings of the National Academy of Sciences of the United States of America·Yuu HiroseMasahiko Ikeuchi
Oct 11, 2008·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Masahiko Ikeuchi, Takami Ishizuka
Oct 11, 2008·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Rei NarikawaMasahiko Ikeuchi
Sep 2, 2009·Proceedings of the National Academy of Sciences of the United States of America·Xiaojing YangKeith Moffat
Feb 16, 2010·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Nathan C Rockwell, J Clark Lagarias
Dec 31, 2010·Nature Methods·Karl Deisseroth
Jun 15, 2011·Proceedings of the National Academy of Sciences of the United States of America·Ji-Young SongYoun-Il Park
Jun 30, 2011·Proceedings of the National Academy of Sciences of the United States of America·Nathan C RockwellJ Clark Lagarias
Apr 13, 2012·Biochemistry·Nathan C RockwellJ Clark Lagarias
Nov 16, 2012·Biochemistry·Nathan C RockwellJ Clark Lagarias
Dec 21, 2012·Proceedings of the National Academy of Sciences of the United States of America·Rei NarikawaMasahiko Ikeuchi
Jul 17, 2013·Proceedings of the National Academy of Sciences of the United States of America·Adam N Bussell, David M Kehoe
Dec 18, 2013·The Journal of Biological Chemistry·Claudia C CornilescuRichard D Vierstra
Jun 4, 2014·Proceedings of the National Academy of Sciences of the United States of America·Carlos GasserAndreas Möglich
Jul 2, 2014·Proceedings of the National Academy of Sciences of the United States of America·Min-Hyung RyuMark Gomelsky
Jul 26, 2014·The Journal of Biological Chemistry·Gen EnomotoMasahiko Ikeuchi
Oct 25, 2014·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Nathan C RockwellJ Clark Lagarias
Apr 22, 2015·Biochemical and Biophysical Research Communications·Rei NarikawaMasahiko Ikeuchi
Jun 17, 2015·Proceedings of the National Academy of Sciences of the United States of America·Gen EnomotoMasahiko Ikeuchi
Jul 3, 2015·Frontiers in Molecular Biosciences·Thea Ziegler, Andreas Möglich
Aug 5, 2015·Biochimica Et Biophysica Acta·Chavdar SlavovJosef Wachtveitl
Aug 5, 2015·Current Opinion in Structural Biology·Katrin Anders, Lars-Oliver Essen

❮ Previous
Next ❯

Citations

Apr 11, 2018·The Journal of Biological Chemistry·Matthew Blain-HartungJ Clark Lagarias
Aug 14, 2020·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Keiji FushimiRei Narikawa
May 14, 2017·Photochemistry and Photobiology·Keiji FushimiRei Narikawa
Apr 6, 2019·Proceedings of the National Academy of Sciences of the United States of America·Keiji FushimiRei Narikawa
Jun 24, 2020·Proceedings of the National Academy of Sciences of the United States of America·Keiji FushimiRei Narikawa
Aug 4, 2018·International Journal of Molecular Sciences·Xian-Jun WuPing-Ping Li
Jan 6, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Sean R TachibanaChong Fang
Nov 19, 2020·Journal of Microbiology and Biotechnology·Su-Dan JiangPing-Ping Li
Mar 18, 2021·Proceedings of the National Academy of Sciences of the United States of America·Sepalika BandaraXiaojing Yang
Apr 21, 2021·Physical Chemistry Chemical Physics : PCCP·Aditya G RaoIgor Schapiro

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.