Distinctive Regulation of Carbapenem Susceptibility in Pseudomonas aeruginosa by Hfq.

Frontiers in Microbiology
Elisabeth SonnleitnerUdo Bläsi

Abstract

Carbapenems are often the antibiotics of choice to combat life threatening infections caused by the opportunistic human pathogen Pseudomonas aeruginosa. The outer membrane porins OprD and OpdP serve as entry ports for carbapenems. Here, we report that the RNA chaperone Hfq governs post-transcriptional regulation of the oprD and opdP genes in a distinctive manner. Hfq together with the recently described small regulatory RNAs (sRNAs) ErsA and Sr0161 is shown to mediate translational repression of oprD, whereas opdP appears not to be regulated by sRNAs. At variance, our data indicate that opdP is translationally repressed by a regulatory complex consisting of Hfq and the catabolite repression protein Crc, an assembly known to be key to carbon catabolite repression in P. aeruginosa. The regulatory RNA CrcZ, which is up-regulated during growth of P. aeruginosa on less preferred carbon sources, is known to sequester Hfq, which relieves Hfq-mediated translational repression of genes. The differential carbapenem susceptibility during growth on different carbon sources can thus be understood in light of Hfq-dependent oprD/opdP regulation and of the antagonizing function of the CrcZ RNA on Hfq regulatory complexes.

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Citations

Sep 22, 2020·Trends in Genetics : TIG·Daniel G MediatiJai J Tree
Aug 28, 2021·International Journal of Molecular Sciences·Petra PusicUdo Bläsi
Aug 28, 2021·Frontiers in Microbiology·Brice Felden, Yoann Augagneur

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Datasets Mentioned

BETA
PRJEB37368

Methods Mentioned

BETA
GRIL-seq
ChIP-seq
PCR
in vitro transcription
microscale thermophoresis
Assay
RNAseq
co-immunoprecipitation assay
RNA-seq
co-immunoprecipitation

Software Mentioned

ImageLab
NTAffinityAnalysis
ViennaNGS
UCSC Genome Browser
Excel
Genome Browser

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Carbapenems are members of the beta lactam class of antibiotics and are used for the treatment of severe or high-risk bacterial infections. Discover the latest research on carbapenems here.

Carbapenems

Carbapenems are members of the beta lactam class of antibiotics and are used for the treatment of severe or high-risk bacterial infections. Discover the latest research on carbapenems here.