Distinguishing Mast Cell Progenitors from Mature Mast Cells in Mice

Stem Cells and Development
Joakim S DahlinJenny Hallgren

Abstract

Mast cells originate from the bone marrow and develop into c-kit(+) FcɛRI(+) cells. Both mast cell progenitors (MCp) and mature mast cells express these cell surface markers, and ways validated to distinguish between the two maturation forms with flow cytometry have been lacking. Here, we show that primary peritoneal MCp from naïve mice expressed high levels of integrin β7 and had a low side scatter (SSC) light profile; whereas mature mast cells expressed lower levels of integrin β7 and had a high SSC light profile. The maturation statuses of the cells were confirmed using three main strategies: (1) MCp, but not mature mast cells, were shown to be depleted by sublethal whole-body γ-irradiation. (2) The MCp were small and immature in terms of granule formation, whereas the mature mast cells were larger and had fully developed metachromatic granules. (3) The MCp had fewer transcripts of mast cell-specific proteases and the enzyme responsible for sulfation of heparin than mature mast cells. Moreover, isolated peritoneal MCp gave rise to mast cells when cultured in vitro. To summarize, we have defined MCp and mature mast cells in naïve mice by flow cytometry. Using this strategy, mast cell maturation can be studied in vivo.

References

Jan 3, 2001·Proceedings of the National Academy of Sciences of the United States of America·C Li, W H Wong
Nov 7, 2001·The Journal of Experimental Medicine·M F GurishK F Austen
Feb 12, 2002·Bioinformatics·Alexander SturnZlatko Trajanoski
Jun 16, 2005·The Journal of Experimental Medicine·Charlotte L WellerTimothy J Williams
Jul 12, 2005·Proceedings of the National Academy of Sciences of the United States of America·Ching-Cheng ChenStephen J Galli
Dec 7, 2005·Proceedings of the National Academy of Sciences of the United States of America·Yojiro ArinobuKoichi Akashi
Dec 14, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jenny HallgrenMichael F Gurish
Aug 10, 2011·Proceedings of the National Academy of Sciences of the United States of America·Wei XingTatiana G Jones
Sep 14, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Joakim S DahlinJenny Hallgren
Mar 7, 2014·Molecular Immunology·Joakim S Dahlin, Jenny Hallgren

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Citations

Aug 10, 2017·Blood·Joakim S DahlinGunnar Nilsson
Feb 17, 2018·Médecine sciences : M/S·Fabien Milliat, Agnès François
Dec 13, 2019·Clinical Reviews in Allergy & Immunology·Daniel Elieh Ali KomiLeonard Bielory
Apr 14, 2018·Journal of Immunology Research·Angélica Aponte-LópezSamira Muñoz-Cruz
Sep 6, 2018·Nature Reviews. Cardiology·Elvira ForteNadia Rosenthal
Jul 21, 2017·Clinical Reviews in Allergy & Immunology·Daniel Elieh Ali KomiStefan Wöhrl
Apr 25, 2019·International Journal of Molecular Sciences·Alan V Nguyen, Athena M Soulika
Jun 14, 2019·Frontiers in Immunology·Erika Méndez-Enríquez, Jenny Hallgren
Mar 30, 2020·Journal of Neuroinflammation·Marian Jesabel Pérez-RodríguezClaudia González-Espinosa
Sep 13, 2018·Blood Advances·Jennine GrootensJoakim S Dahlin
Nov 16, 2019·PLoS Biology·Toshihiko KobayashiNoriko Toyama-Sorimachi
Nov 27, 2019·Cells·Ralf WeiskirchenMichael Huber
Mar 17, 2020·Frontiers in Immunology·Maya SalomonssonJenny Hallgren
Nov 21, 2020·Communications Biology·Andrew P AhYoungMenno van Lookeren Campagne
Dec 29, 2020·Current Opinion in Allergy and Clinical Immunology·Ryan C Murphy, Teal S Hallstrand
Mar 2, 2021·Frontiers in Immunology·Viktor BugajevPetr Draber
Jul 3, 2021·Frontiers in Immunology·Mariela JiménezEva Salinas
Aug 27, 2021·Drug Discovery Today·Sherin SaheeraRajarajan A Thandavarayan
Sep 8, 2021·The Journal of Allergy and Clinical Immunology·Erika Méndez-EnríquezJenny Hallgren

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Datasets Mentioned

BETA
GSE63507

Methods Mentioned

BETA
flow cytometry
lavage
fluorescence-activated cell sorting
FACS

Software Mentioned

NIS Elements AR
NIS Elements BR
GraphPad
ImageJ
Graphpad Prism
FlowJo

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