PMID: 9440347Jan 24, 1998Paper

Distortion correction of tubular crystals: improvements in the acetylcholine receptor structure

Ultramicroscopy
R Beroukhim, N Unwin

Abstract

Biological molecules often crystallize either as tubes, having helical symmetry, or as two-dimensional sheets. Both sorts of crystal are potentially suitable for structure determination to atomic resolution by electron crystallography, but their lattice distortions must first be corrected. We have developed a procedure for tubular crystals, based on independent alignment of very short segments against a reference structure, that allows accurate determination and correction of distortions in all three dimensions. Application of this procedure to images used previously to determine the 9 A structure of the acetylcholine receptor showed that about half of the signal loss caused by the distortions arises from effects correctable in the image plane (bending, changes in scale) and half from effects requiring out-of-plane correction (variations in tilt and in twist around the tube axis). By dividing the tubes into short segments (of lengths about equal to their diameter) it became possible to recover almost all of this loss without reducing appreciably the accuracy in the segmental alignments. The signal retention improved by only 10% at low resolution (20 A), but by progressively greater amounts at higher resolutions, up to approxima...Continue Reading

References

Oct 1, 1992·Ultramicroscopy·D G Morgan, D DeRosier
Jun 20, 1990·Journal of Molecular Biology·R HendersonK H Downing
Jan 5, 1989·Journal of Molecular Biology·T W JengW Chiu
Jan 1, 1988·Ultramicroscopy·D A BluemkeR Josephs
Dec 14, 1995·Nature·J D JontesR A Milligan
Jun 1, 1995·Ultramicroscopy·M WhittakerR A Milligan

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Citations

Nov 18, 1998·Current Opinion in Structural Biology·M H StowellN Unwin
Feb 13, 2001·Micron : the International Research and Review Journal for Microscopy·G Mosser
Oct 6, 1999·Current Opinion in Chemical Biology·W Kühlbrandt, K A Williams
Jan 24, 2013·Proceedings of the National Academy of Sciences of the United States of America·Nicolas CoudrayDavid L Stokes
Jun 4, 2004·The EMBO Journal·Naoko MizunoMasahide Kikkawa
Apr 29, 2015·Archives of Biochemistry and Biophysics·Edward H Egelman
Jul 31, 2012·Journal of Molecular Biology·Nigel Unwin, Yoshinori Fujiyoshi
Nov 26, 2013·Journal of Structural Biology·Ambroise DesfossesCarsten Sachse
Aug 30, 2008·Current Opinion in Structural Biology·Yoshinori Fujiyoshi, Nigel Unwin
Jun 6, 1998·Journal of Structural Biology·N Unwin
Jun 4, 2016·Journal of Structural Biology·Nicolas CoudrayDavid L Stokes
Oct 22, 2016·Protein Science : a Publication of the Protein Society·Nicolas CoudrayDavid L Stokes
Jan 29, 2002·Journal of Molecular Biology·Galen M HandGina E Sosinsky
Feb 11, 2005·Journal of Molecular Biology·Nigel Unwin
Oct 5, 2001·Nature Cell Biology·P Zhang, J E Hinshaw
Feb 24, 2001·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·N Unwin
Jun 27, 2003·Nature·Atsuo MiyazawaNigel Unwin
Feb 16, 2019·Journal of Neurosciences in Rural Practice·Prerna GargSanjay Dhall
Feb 11, 2020·Acta Crystallographica. Section D, Structural Biology·Sjors H W Scheres
Mar 1, 2005·The Journal of Biological Chemistry·David L StokesJean-Jacques Lacapère
Apr 10, 2020·Neuropharmacology·Anant GharpureRyan E Hibbs
May 1, 2001·Journal of Molecular Biology·H S YoungD L Stokes
Feb 7, 2002·Journal of Molecular Biology·Chen XuDavid L Stokes
Jun 26, 2007·Journal of Molecular Biology·Carsten SachseNikolaus Grigorieff
Sep 17, 2011·Chemical Reviews·E V Orlova, H R Saibil

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