Mar 1, 1976

Distribution and biliary excretion products of di-2-ethylhexyl phthalate in rainbow trout

Drug Metabolism and Disposition : the Biological Fate of Chemicals
M J Melancon, J J Lech


After 24-hr exposures of rainbow trout to 0.5 ppm of di-2-ethylhexyl [14C]phthalate, one-half of the radioactivity present in the fish was localized in the bile. The bile was pooled and fractionated by selective solvent extraction before and after beta-glucuronidase hydrolysis. Individual radioactive compounds were further separated and characterized by thin-layer chromatography. Gas chromatography-mass spectrometry was used to confirm the results of thin-layer chromatographic analysis. These procedures demonstrated that bile contained a number of DEHP metabolites, but only about 1% of unchanged DEHP. The major metabolite, mono-2-ethylhexyl phthalate glucuronide accounted for 72% of the total bile radioactivity. The remaining bile radioactivity was found to be present as phthalic acid glucuronide, mono-2-ethylhexyl phthalate and two partially characterized polar metabolites.

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Mentioned in this Paper

Diethylhexyl Phthalate
Tissue Specificity
Glucuronic Acids
Phthalic Acids
Biliary Sludge
Chromatography, Gas-Liquid-Mass Spectrometry
Thin Layer Chromatography

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