PMID: 8950504Nov 1, 1996Paper

Distribution and characterization of proteoglycans associated with exfoliation material

Current Eye Research
A TawaraH Inomata

Abstract

To examine the distribution of proteoglycans in the exfoliation materials in order to investigate the nature of the materials. The anterior parts of two eyes with exfoliation syndrome were examined by electron microscopy after staining with cupromeronic blue (cmb). Some specimens were treated with enzymes and/or nitrous acid prior to staining. The effects of the enzymes were evaluated statistically by counting the density of the cmb-positive filaments in the exfoliation materials, using a computer. One eye with exfoliation syndrome stained with alcian blue was observed with light microscopy. Exfoliation materials were observed along the epithelial cells of the iris and ciliary body, and in the trabecular meshwork and zonules. In tissue specimens treated with cmb, electron-dense filaments were seen associated with the exfoliation materials. Microfibrils in the trabecular meshwork and iris, and zonular fibrils themselves were free of any filament staining, while the exfoliation materials located closely to the fibrils contained the electron-dense filaments. In the tissue specimens treated with chondroitinase AC, chondroitinase B, chondroitinase ABC or nitrous acid before cmb staining, the amount of the filament associated with ex...Continue Reading

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Citations

Aug 10, 2011·Archives of Ophthalmology·Joshua D SteinJulia E Richards
Nov 1, 2005·Biochimica Et Biophysica Acta·M AssoutiS P Gartaganis
Mar 30, 2018·Journal of Glaucoma·Tero T Kivelä
Oct 3, 2014·Journal of Glaucoma·Robert Ritch
Oct 3, 2014·Journal of Glaucoma·Margaret E HuflejtMarko I Vuskovic
Feb 13, 2001·Survey of Ophthalmology·R Ritch, U Schlötzer-Schrehardt

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