Distribution and colocalisation of glutamate decarboxylase isoforms in the rat spinal cord

Neuroscience
M MackieA J Todd

Abstract

The inhibitory neurotransmitter GABA is synthesized by glutamic acid decarboxylase (GAD), and two isoforms of this enzyme exist: GAD65 and GAD67. Immunocytochemical studies of the spinal cord have shown that whilst both are present in the dorsal horn, GAD67 is the predominant form in the ventral horn. The present study was carried out to determine the pattern of coexistence of the two GAD isoforms in axonal boutons in different laminae of the cord, and also to examine the relation of the GADs to the glycine transporter GLYT2 (a marker for glycinergic axons), since many spinal neurons are thought to use GABA and glycine as co-transmitters. Virtually all GAD-immunoreactive boutons throughout the spinal grey matter were labelled by both GAD65 and GAD67 antibodies; however, the relative intensity of staining with the two antibodies varied considerably. In the ventral horn, most immunoreactive boutons showed much stronger labelling with the GAD67 antibody, and many of these were also GLYT2 immunoreactive. However, clusters of boutons with high levels of GAD65 immunoreactivity were observed in the motor nuclei, and these were not labelled with the GLYT2 antibody. In the dorsal horn, some GAD-immunoreactive boutons had relatively high...Continue Reading

References

Dec 1, 1975·The Journal of Comparative Neurology·B J McLaughlinJ Y Wu
Nov 1, 1992·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·I J Llewellyn-Smith, J B Minson
Mar 1, 1991·Neurochemical Research·M G Erlander, A J Tobin
Apr 29, 1982·Brain Research·R P BarberE Roberts
Jul 17, 1998·Science·P JonasJ Sandkühler
Jan 1, 1999·Trends in Pharmacological Sciences·J J Soghomonian, D L Martin

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Citations

Jul 15, 2005·Journal of Neurophysiology·Kimberly J DoughertyShawn Hochman
Feb 3, 2012·Physiological Reviews·Hanns Ulrich ZeilhoferGonzalo E Yévenes
Mar 17, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·B Anne BannatyneDavid J Maxwell
Jun 11, 2005·Proceedings of the National Academy of Sciences of the United States of America·D I HughesA J Todd
Dec 11, 2013·The Journal of Physiology·Pradeep PunnakkalHanns Ulrich Zeilhofer
Jan 11, 2011·Neuropharmacology·Young S Gwak, Claire E Hulsebosch
Aug 25, 2007·The Journal of Physiology·David J MaxwellRichard Morris
May 1, 2007·Biochemical and Biophysical Research Communications·Boyoung LeeJin Woo Chang
Mar 6, 2007·Brain Research·Mehdi HossainiJan C Holstege
Mar 17, 2006·Journal of Neurochemistry·Anant B PatelKevin L Behar
Sep 10, 2008·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Jing HuangShengxi Wu
Mar 8, 2013·European Journal of Pain : EJP·M GassnerJ Sandkühler
Dec 18, 2012·Neuromodulation : Journal of the International Neuromodulation Society·Camilla UlteniusBengt Linderoth
Mar 28, 2013·Annals of the New York Academy of Sciences·Lea Ziskind-Conhaim
Jun 9, 2006·Brain Research Reviews·Schahram Akbarian, Hsien-Sung Huang
Nov 19, 2003·The European Journal of Neuroscience·B Anne BannatyneDavid J Maxwell
Apr 22, 2005·The Journal of Comparative Neurology·Chun-Rong LuAldo Rustioni
Nov 24, 2004·The Journal of Comparative Neurology·Mihály PetkóMiklós Antal
Jan 20, 2006·The European Journal of Neuroscience·Yu WangXiang Yang Chen
Oct 29, 2005·The Journal of Comparative Neurology·Francisco J AlvarezMartyn Goulding
Sep 5, 2009·The Journal of Comparative Neurology·Carlos Ernesto RestrepoOle Kiehn
Jul 29, 2008·The European Journal of Neuroscience·Shreejith PillaiXiang Yang Chen
Aug 14, 2010·The European Journal of Neuroscience·Yasmina B MartinAngel Nuñez
Apr 29, 2015·Journal of Neuroscience Research·Maria M Spruill, Ralph W Kuncl
Nov 2, 2014·Journal of Peptide Science : an Official Publication of the European Peptide Society·Selin AgcaNicole Hartwig Trier

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