Distribution and dynamics of rat basophilic leukemia immunoglobulin E receptors (FcepsilonRI) on planar ligand-presenting surfaces.

Biophysical Journal
Kathrin SpendierJames L Thomas

Abstract

There is considerable interest in the signaling mechanisms of immunoreceptors, especially when triggered with membrane-bound ligands. We have quantified the spatiotemporal dynamics of the redistribution of immunoglobulin E-loaded receptors (IgE-FcepsilonRI) on rat basophilic leukemia-2H3 mast cells in contact with fluid and gel-phase membranes displaying ligands for immunoglobulin E, using total internal reflection fluorescence microscopy. To clearly separate the kinetics of receptor redistribution from cell spreading, and to precisely define the initial contact time (+/-50 ms), micropipette cell manipulation was used to bring individual cells into contact with surfaces. On ligand-free surfaces, there are micron-scale heterogeneities in fluorescence that likely reflect regions of the cell that are more closely apposed to the substrate. When ligands are present, receptor clusters form with this same size scale. The initial rate of accumulation of receptors into the clusters is consistent with diffusion-limited trapping with D approximately 10(-1) microm2/s. These results support the hypothesis that clusters form by diffusion to cell-surface contact regions. Over longer timescales (>10 s), individual clusters moved with both diff...Continue Reading

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Citations

Sep 4, 2012·Journal of Biological Physics·Kathrin Spendier, James L Thomas
Sep 23, 2014·PeerJ·Roshni S KalkurKathrin Spendier
Aug 26, 2015·The Journal of Cell Biology·Amanda Carroll-PortilloDiane S Lidke
Jul 28, 2015·Biomaterials Science·Philipp R SpycherErik Reimhult
Feb 4, 2014·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·K SpendierV M Kenkre
Jul 26, 2013·The Journal of Physical Chemistry. B·Kathrin Spendier, V M Kenkre

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