Distribution and immunohistochemical characterization of torsinA immunoreactivity in rat brain

Brain Research
R H WalkerP Shashidharan

Abstract

A mutation of the DYT1 gene on chromosome 9q34 has recently been identified as the cause of one form of autosomal-dominantly inherited dystonia. TorsinA, the protein product of this gene, has homology with the family of heat shock proteins, and is found in many peripheral tissues and brain regions. We used a polyclonal antibody to torsinA, developed in our laboratory, to systematically examine the regional distribution of torsinA in rat brain. We find that neurons in all examined structures are immunoreactive for this protein. There is intense immunoreactivity in most neuronal nuclei, with slightly less labeling of cytoplasm and proximal processes. Terminals also are labeled, especially in striatum, neocortex and hippocampus. Double-labeling fluorescence immunohistochemistry using antibodies to neurotransmitters and other neurochemical markers demonstrated that the majority of neurons of all studied neurochemical types are immunoreactive for torsinA. Our findings indicate that torsinA is widely distributed in the central nervous system implicating additional, localized factors, perhaps within the basal ganglia, in the development of dystonia. Many other proteins have a similar widespread distribution, including some which have ...Continue Reading

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Citations

Dec 4, 2001·Brain Research·M Konakova, S M Pulst
May 15, 2002·Brain Research. Molecular Brain Research·Philipp ZieferSarah J Augood
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May 6, 2017·IEEE Transactions on Neural Systems and Rehabilitation Engineering : a Publication of the IEEE Engineering in Medicine and Biology Society·Yulin ZhuChen Liu
Sep 23, 2003·Movement Disorders : Official Journal of the Movement Disorder Society·Ruth H WalkerP Shashidharan
Sep 30, 2004·Annals of Neurology·Kevin St P McNaughtC Warren Olanow
Nov 19, 2004·Human Molecular Genetics·P ShashidharanC W Olanow

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