Distribution and immunohistochemical characterization of primary afferent neurons innervating the levator ani muscle of the female squirrel monkey

American Journal of Obstetrics and Gynecology
Lisa M PierceKarl B Thor

Abstract

This study was undertaken to examine the neurofilament and neurochemical composition of subpopulations of primary afferent neurons innervating the levator ani muscle by combining retrograde tracing and triple labeling immunofluorescence in the female squirrel monkey. Cholera toxin B subunit (CTB) was injected unilaterally into the levator ani muscle of 3 monkeys to identify primary sensory neurons in the dorsal root ganglia (DRG) and their central projections in the spinal cord. L7-S2 DRG were processed for dual or triple labeling immunofluorescence 3 days after injection to examine labeling of the 200 kD neurofilament marker RT97 (a marker of myelinated neurons), calcitonin gene-related peptide (CGRP; a marker of peptidergic neurons), isolectin B4 (IB4; a marker of small, unmyelinated neurons), and nerve growth factor receptor (TrkA) in CTB-positive neurons. RT97-negative (C-fiber) neurons were more numerous (74% of total CTB-labeled neurons) and smaller in size than RT97-positive (A-fiber) afferent neurons (26% of CTB-labeled neurons). IB4 labeling was almost exclusively found in RT97-negative afferent neurons. Approximately 43% of all CTB-labeled DRG neurons expressed CGRP, and the majority of these were small. The distribut...Continue Reading

References

Oct 8, 1989·The Journal of Comparative Neurology·K B ThorW C De Groat
Apr 1, 1986·Canadian Journal of Physiology and Pharmacology·V C Abrahams
Jan 1, 1995·Somatosensory & Motor Research·J E SwettP G Miller
Nov 1, 2001·Brain Research. Molecular Brain Research·H KashibaE Senba
May 16, 2003·American Journal of Obstetrics and Gynecology·Lisa M PierceKimberly W Coates
Feb 1, 1946·The Anatomical Record·M ABERCROMBIE

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Citations

May 14, 2011·International Urogynecology Journal·Jessica N BrackenThomas J Kuehl
May 21, 2010·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Karl B Thor, William C de Groat
Aug 24, 2012·Journal of Neurophysiology·Pedro AlvarezJon D Levine
Jan 22, 2008·Molecular and Cellular Neurosciences·Valerio MagnaghiBernhard Bettler
Sep 15, 2015·American Journal of Obstetrics and Gynecology·Fiona M LindoWilma I Larsen
Sep 3, 2009·Microscopy Research and Technique·Domenico RussoGiovanna Lalatta-Costerbosa
Dec 22, 2009·Neurourology and Urodynamics·L BirderM Drake
Mar 4, 2008·American Journal of Obstetrics and Gynecology·Lisa M PierceThomas J Kuehl
Dec 31, 2011·Experimental Neurology·Pedro AlvarezJon D Levine
Nov 10, 2013·PloS One·Alexander A TarnutzerSarah Marti
Jul 19, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·D D RahnR A Word
Feb 23, 2020·Muscle & Nerve·Satoshi OgaMinoru Okita
Jul 3, 2017·Journal of Neuroscience Methods·Simone GrossKaspar Matiasek

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