PMID: 3756771Nov 15, 1986Paper

Distribution and modulation of surface charges of cells from human leukemia-lymphoma lines at various stages of differentiation

Cancer
Y MarikovskyH Ben-Bassat

Abstract

Untreated and retinoic acid (RA) treated human leukemia-lymphoma cell lines reflecting hematopoietic cells at various stages of differentiation, were examined electron microscopically for their surface negative charge distribution using cationized ferritin (CF), an electron dense label of anionic sites. The results indicate that there is a correlation between the CF labeling density/distribution and the stage of lymphoid cell differentiation. Viable unfixed null cell lines show a low CF labeling density with few and small CF patches. A gradual increase in CF labeling density and increase in size and number of CF patches correlates with the stage of differentiation on cell lines of both T or B origin. Treatment of viable unfixed cells with 10(-5) MRA for 10 days seems to prevent the CF-induced formation of CF patches, resulting in a continuous and even distribution of the CF label, similar to that observed on the surface of cells fixed before CF labeling. Some correlation between the distribution of surface anionic sites and the malignant potential of the human leukemic lines could be detected.

References

Feb 15, 1976·International Journal of Cancer. Journal International Du Cancer·K O Fresen, H Hausen
Apr 1, 1978·Journal of the National Cancer Institute·Y MarikovskyN Reichman
Mar 1, 1977·Journal of the National Cancer Institute·L D DionG E Gifford
Mar 1, 1972·Journal of Ultrastructure Research·D DanonE Skutelsky
May 15, 1980·International Journal of Cancer. Journal International Du Cancer·H Ben-BassatA Polliack
Jan 1, 1983·Experimental Cell Biology·Y MarikovskyM Inbar
May 1, 1965·Journal of Clinical Pathology·J V PULVERTAFT
Jun 15, 1980·International Journal of Cancer. Journal International Du Cancer·Z RavidM Greaves

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