Distribution and Phylogeny of Microsymbionts Associated with Cowpea (Vigna unguiculata) Nodulation in Three Agroecological Regions of Mozambique

Applied and Environmental Microbiology
Ifeoma N ChidebeFelix D Dakora

Abstract

Cowpea derives most of its N nutrition from biological nitrogen fixation (BNF) via symbiotic bacteroids in root nodules. In Sub-Saharan Africa, the diversity and biogeographic distribution of bacterial microsymbionts nodulating cowpea and other indigenous legumes are not well understood, though needed for increased legume production. The aim of this study was to describe the distribution and phylogenies of rhizobia at different agroecological regions of Mozambique using PCR of the BOX element (BOX-PCR), restriction fragment length polymorphism of the internal transcribed spacer (ITS-RFLP), and sequence analysis of ribosomal, symbiotic, and housekeeping genes. A total of 122 microsymbionts isolated from two cowpea varieties (IT-1263 and IT-18) grouped into 17 clades within the BOX-PCR dendrogram. The PCR-ITS analysis yielded 17 ITS types for the bacterial isolates, while ITS-RFLP analysis placed all test isolates in six distinct clusters (I to VI). BLASTn sequence analysis of 16S rRNA and four housekeeping genes (glnII, gyrB, recA, and rpoB) showed their alignment with Rhizobium and Bradyrhizobium species. The results revealed a group of highly diverse and adapted cowpea-nodulating microsymbionts which included Bradyrhizobium pa...Continue Reading

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Citations

Oct 9, 2018·Frontiers in Microbiology·Jann Lasse Grönemeyer, Barbara Reinhold-Hurek
Jun 19, 2020·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Haruna MohammedFelix D Dakora
Mar 12, 2019·Frontiers in Microbiology·Sanjay K Jaiswal, Felix D Dakora
May 22, 2021·Frontiers in Microbiology·Guohua LiuYan Li
Jun 15, 2021·Systematic and Applied Microbiology·Margarida G SimbineFelix D Dakora

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Datasets Mentioned

BETA
KY941240
KY941398

Methods Mentioned

BETA
electrophoresis
PCR
environmental stress

Software Mentioned

NTSYS pc
edit
BLAST
Bio
MEGA
MUSCLE
BioEdit
ClustalW
Numerics

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