Distribution and possible role of perinuclear theca proteins during bovine spermiogenesis

Microscopy Research and Technique
R Oko, D Maravei

Abstract

The perinuclear theca (PT) is a unique cytoskeletal element that encapsulates the mammalian sperm nucleus. It is divided into subacrosomal and postacrosomal regions. The objective of this study was to analyze and stage the intracellular distribution of several prominent bull PT proteins during spermatogenesis. For this purpose, polyclonal antibodies raised and affinity-purified against the 15.5-, 25-, 28-, 32-, 36-, and 60-kDa bull PT polypeptides were used as probes on sections of aldehyde-fixed testes. Immunoperoxidase staining revealed that the PT polypeptides first appeared early in spermiogenesis, concomitant with early steps of development of the acrosomic system. Immunogold labeling further showed that these polypeptides were peripherally associated with the entire acrosomal membrane, before and during the attachment of the acrosomic vesicle onto the spermatid's nucleus. Once the acrosome had capped the nucleus the labeling resided mainly in the subacrosomal region of the spermatid, between the inner acrosomal membrane and nuclear envelope. Later, during the elongation of the spermatid's nucleus, the labeling with all antibodies except the anti-15.5-kDa antibody extended caudally over the assembling postacrosomal sheath....Continue Reading

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Citations

Jan 6, 2001·Molecular Reproduction and Development·M K O'BryanD M De Kretser
Mar 6, 2009·Reproduction, Fertility, and Development·William G BreedRichard J Oko
Apr 19, 2012·Human Reproduction·Cristian Alvarez SedóHéctor E Chemes
Mar 17, 2004·Archives of Histology and Cytology·Kiyotaka Toshimori, Chizuru Ito
Oct 12, 2011·Cell and Tissue Research·Richard OkoAarnoud C van der Spoel
Nov 4, 2009·Journal of Reproductive Immunology·Richard Oko, Peter Sutovsky
Feb 11, 2009·Microscopy Research and Technique·Yang YuRichard Oko
Oct 8, 2005·Fertility and Sterility·Masahito TachibanaKunihiro Okamura
Mar 21, 2003·Biochemical and Biophysical Research Communications·Roselyne Rousseaux-PrévostJean Rousseaux
Nov 25, 2000·Biology of Reproduction·C LécuyerR Rousseaux-Prévost
Apr 11, 2008·Biology of Reproduction·Jeremi R MountjoyRichard Oko
Jan 1, 2005·The Journal of Pharmacology and Experimental Therapeutics·Erik J Martin, Poh-Gek Forkert
Jun 5, 2003·The Journal of Biological Chemistry·P Ronald Tovich, Richard J Oko
Jul 28, 2005·Reproductive Medicine and Biology·Yukihiro TeradaKunihiro Okamura
Oct 7, 2020·Physiology International·Esma MenevseEmine Nedime Korucu

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