Distribution and Quantitative Estimates of Variant Creutzfeldt-Jakob Disease Prions in Tissues of Clinical and Asymptomatic Patients

Emerging Infectious Diseases
Jean Y DouetOlivier Andréoletti

Abstract

In the United-Kingdom, ≈1 of 2,000 persons could be infected with variant Creutzfeldt-Jakob disease (vCJD). Therefore, risk of transmission of vCJD by medical procedures remains a major concern for public health authorities. In this study, we used in vitro amplification of prions by protein misfolding cyclic amplification (PMCA) to estimate distribution and level of the vCJD agent in 21 tissues from 4 patients who died of clinical vCJD and from 1 asymptomatic person with vCJD. PMCA identified major levels of vCJD prions in a range of tissues, including liver, salivary gland, kidney, lung, and bone marrow. Bioassays confirmed that the quantitative estimate of levels of vCJD prion accumulation provided by PMCA are indicative of vCJD infectivity levels in tissues. Findings provide critical data for the design of measures to minimize risk for iatrogenic transmission of vCJD.

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Citations

Dec 1, 2017·PLoS Pathogens·Abigail B DiackJean C Manson
Feb 21, 2018·The Journal of General Virology·Cyrus BettLuisa Gregori
Aug 19, 2020·International Journal of Molecular Sciences·Isabel M GuijarroMarta Monzón
Dec 18, 2019·Proceedings of the National Academy of Sciences of the United States of America·Alvina HuorOlivier Andreoletti
Dec 19, 2020·Frontiers in Bioengineering and Biotechnology·Mohammed MoudjouAngélique Igel-Egalon
Feb 6, 2021·Biomolecules·Diane L Ritchie, Marcelo A Barria
Feb 4, 2021·Acta Neuropathologica·Jean-Yves DouetOlivier Andreoletti

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transgenic

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