Abstract
High-flow microinfusion is a novel technique for delivery of compounds directly into brain parenchyma, bypassing the blood-brain barrier. The feasibility of this technique has been demonstrated with low-molecular-weight compounds, macromolecular dyes, and proteins. Delivery of antisense oligonucleotides into brain parenchyma represents an additional potential application of this technique not previously described. In this report the authors sought to examine the distribution and disposition of phosphorothioate oligodeoxynucleotide (PS-ODN) for this reason. An 18-mer 35S-PS-ODN (Mr approximately 6000) was infused over 1 hour into the caudate putamen of Fischer 344 rats. At 1, 6, 12, 24, and 48 hours after beginning the infusion, the brains were extracted and analyzed using quantitative autoradiographic techniques. Cerebrospinal fluid (CSF) was also aspirated from the cisterna magna and was analyzed to determine the radioactivity and stability of the 35S-PS-ODN. At 1 hour, the infused ODN was uniformly distributed in brain tissue, with a maximum average concentration of 4806.5 +/- 210.5 nCi/g. This represents a tissue concentration of 19.2 +/- 0.84 microM. Extensive spread into surrounding parenchyma was observed over the ensuing...Continue Reading
References
Oct 1, 1992·Brain Pathology·R O WellerE T Zhang
Jan 1, 1992·Acta Neuropathologica·E T ZhangR O Weller
Apr 1, 1992·Biopharmaceutics & Drug Disposition·G GoodarziK Watabe
Sep 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·S AgrawalJ Y Tang
Mar 1, 1990·Journal of Biochemical and Biophysical Methods·J M CampbellE Wickstrom
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S AgrawalP C Zamecnik
Aug 1, 1988·Journal of Biochemical and Biophysical Methods·T A BaconE Wickstrom
Jan 1, 1970·Brain : a Journal of Neurology·L Bakay
Jan 1, 1970·Brain : a Journal of Neurology·L Bakay
Feb 1, 1982·Journal of Neurosurgery·A MarmarouK Shulman
Jun 1, 1995·Journal of Neurosurgery·D M LiebermanE H Oldfield
Oct 1, 1994·Cellular and Molecular Neurobiology·B J ChiassonH A Robertson
Aug 8, 1995·Biochemical Pharmacology·R ZhangS Agrawal
Aug 8, 1995·Biochemical Pharmacology·S AgrawalZ Jiang
Jun 4, 1993·Science·R W WagnerB C Froehler
Apr 11, 1995·Nucleic Acids Research·J Y MalteseR Narayanan
Feb 1, 1995·Current Opinion in Biotechnology·S Agrawal, R P Iyer
Jan 1, 1994·Antisense Research and Development·P L IversenG Zon
Aug 1, 1994·The American Journal of Physiology·S Napathorn, K R Spring
Mar 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·R H BoboE H Oldfield
Jan 1, 1993·Methods in Molecular Biology·B C Froehler
Aug 20, 1993·Science·C A Stein, Y C Cheng
May 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·L WhitesellL M Neckers
Oct 8, 1997·Neurosurgery·W C BroaddusH Fillmore
Citations
Jul 15, 1999·Physiology & Behavior·M C FlynnJ M Ffrench-Mullen
Jul 2, 2005·Oligonucleotides·Reimar SchlingensiepenKarl-Hermann Schlingensiepen
Jul 14, 2011·Neuro-oncology·Adam M SonabendJeffrey N Bruce
Jun 25, 1999·The British Journal of Ophthalmology·W Y ShenP E Rakoczy
Jul 28, 2005·Cerebrospinal Fluid Research·Tavarekere N NagarajaJoseph D Fenstermacher
Jan 31, 2006·Neuro-oncology·Alexandre CarpentierAntoine F Carpentier
Sep 25, 2012·Journal of Neurosurgery·George K LewisWilliam L Olbricht
Sep 25, 2007·Journal of Neurosurgery·Nicholas J SzerlipRussell R Lonser
Feb 9, 1999·Journal of Neurosurgery·M Y ChenE H Oldfield
Aug 18, 2004·Journal of Neurosurgery·Zhi-Jian ChenPanos P Fatouros
Sep 24, 2005·Journal of Neurosurgery·Michael Y ChenEdward H Oldfield
Jul 1, 2006·Neurological Research·Kim A LopezJeffrey N Bruce
Jan 23, 2014·Journal of Medical Engineering & Technology·P J HaarW C Broaddus
Feb 17, 2007·Neuroscience·Neelima B Chauhan, George J Siegel
May 9, 2001·IEEE Transactions on Bio-medical Engineering·P J HaarW C Broaddus
Jun 18, 2002·IEEE Transactions on Bio-medical Engineering·Zhi-Jian ChenGeorge T Gillies
May 25, 2006·Surgical Neurology·Shenghua ChuJie Zhang
Jul 28, 2015·Nucleic Acid Therapeutics·Yanyan CenHong Zhou
Oct 19, 2005·Peptides·William A BanksJohn E Morley
Apr 27, 2001·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·N CholetG Bonvento
Jul 24, 2001·Anesthesiology·C L WuJ Yang
Aug 27, 1999·Antisense & Nucleic Acid Drug Development·S SeidmanH Soreq
May 13, 2011·Neurosurgery·Jeffrey N BruceRobert L DeLaPaz
Oct 12, 1999·The American Journal of Physiology·P F MorrisonE H Oldfield
Aug 31, 2006·Journal of Neuro-oncology·Yang LiuKevin O Lillehei
Aug 17, 1999·Neurosurgery·M R BullockJ P Muizelaar
Mar 17, 2000·Neurosurgery·J N BruceA T Parsa