Distribution and viral load of type specific HPVs in different cervical lesions as detected by PCR-ELISA

Journal of Clinical Pathology
Marialuisa ZerbiniMonica Musiani

Abstract

To investigate the distribution and viral load of the most prevalent high risk human papillomavirus (HPV) types 16, 18, 31, 33, and 45 and low risk HPV types 6 and 11 in a variety of cervical lesions. One hundred and seventy six cytological specimens from women with different cervical lesions were investigated. For an accurate standardisation of the sample, cervical cells were counted and a volume of the cell suspension processed by polymerase chain reaction-enzyme linked immunosorbent assay (PCR-ELISA). Semiquantitative determinations were achieved in relation to an external reference titration curve. HPV DNA was detected in 60.2% of the samples. HPV-16 was the prevalent genotype (57.6%), followed by HPV-33, HPV-31, HPV-6, HPV-18, and HPV-45. HPV-11 was not detected. HPV-16 showed a pronounced increase in prevalence with the evolution of cervical disease. Semiquantitative evaluation of the results showed that only HPV-16 DNA could reach very high values (> 1000 genome copies/cell) and a very high HPV-16 load correlated with the severity of cervical disease. Only HPV-16 load appears to be associated with the severity of cervical disease.

Citations

Oct 9, 2002·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Simona VenturoliMarialuisa Zerbini
Jan 30, 2004·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·M CriccaM Zerbini
Jan 30, 2004·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Joséphine AhoFrancois Coutlée
Oct 20, 2007·Nature Protocols·Monica MusianiMarialuisa Zerbini
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Dec 23, 2008·Medicinski pregled·Milana Panjković, Tatjana Ivković-Kapicl
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Feb 4, 2009·Journal of Virological Methods·Monica CriccaMarialuisa Zerbini
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Apr 18, 2015·BMC Cancer·Luisa Del Río-OspinaManuel Alfonso Patarroyo
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Jul 23, 2003·Archives of Pathology & Laboratory Medicine·Roger A Hubbard

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