Distribution, mobility, and anchoring of lignin-related oxidative enzymes in Arabidopsis secondary cell walls

Journal of Experimental Botany
Eva Yi ChouA L Samuels

Abstract

Lignin is an important phenolic biopolymer that provides strength and rigidity to the secondary cell walls of tracheary elements, sclereids, and fibers in vascular plants. Lignin precursors, called monolignols, are synthesized in the cell and exported to the cell wall where they are polymerized into lignin by oxidative enzymes such as laccases and peroxidases. In Arabidopsis thaliana, a peroxidase (PRX64) and laccase (LAC4) are shown to localize differently within cell wall domains in interfascicular fibers: PRX64 localizes to the middle lamella whereas LAC4 localizes throughout the secondary cell wall layers. Similarly, laccases localized to, and are responsible for, the helical depositions of lignin in protoxylem tracheary elements. In addition, we tested the mobility of laccases in the cell wall using fluorescence recovery after photobleaching. mCHERRY-tagged LAC4 was immobile in secondary cell wall domains, but mobile in the primary cell wall when ectopically expressed. A small secreted red fluorescent protein (sec-mCHERRY) was engineered as a control and was found to be mobile in both the primary and secondary cell walls. Unlike sec-mCHERRY, the tight anchoring of LAC4 to secondary cell wall domains indicated that it canno...Continue Reading

References

Jan 1, 1990·The Plant Cell·J DeneckeR Deblaere
Dec 11, 2002·European Journal of Biochemistry·Karen G WelinderLaurent Duroux
Jun 1, 1996·Annual Review of Plant Physiology and Plant Molecular Biology·Hiroo Fukuda
Aug 17, 2005·Genes & Development·Minoru KuboTaku Demura
Jun 14, 2008·Plant Physiology·Minako KanedaA Lacey Samuels
Mar 7, 2009·Journal of Experimental Botany·Kaisa MarjamaaKurt V Fagerstedt
May 18, 2010·Plant Physiology·Ruben VanholmeWout Boerjan
Feb 3, 2011·The Plant Journal : for Cell and Molecular Biology·Masatoshi YamaguchiTaku Demura
Jul 19, 2011·The New Phytologist·Alexandre MartinièreHarriet G McWatters
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Sep 1, 1988·Planta·O Baron-EpelM Schindler
Feb 20, 2014·Current Opinion in Biotechnology·Xianzhi Meng, Arthur Jonas Ragauskas
Apr 1, 2014·Current Opinion in Biotechnology·Emilie A Rennie, Henrik Vibe Scheller
Aug 27, 2014·Plant Physiology·Mathias SchuetzA Lacey Samuels
Apr 17, 2015·Annals of Botany·Jaime BarrosEdouard Pesquet
Nov 7, 2015·The New Phytologist·Jun Shigeto, Yuji Tsutsumi
Sep 21, 2016·Proceedings of the National Academy of Sciences of the United States of America·Shundai LiYing Gu
Dec 22, 2016·Nature Communications·Thomas J SimmonsPaul Dupree
Jan 20, 2017·Biotechnology for Biofuels·Gabriel PaësBrigitte Chabbert
Apr 19, 2017·Plant Physiology·Rebecca A SmithA Lacey Samuels

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Citations

Oct 30, 2019·Proceedings of the National Academy of Sciences of the United States of America·Josh V VermaasGregg T Beckham
Dec 5, 2019·Open Biology·Richard A Dixon, Jaime Barros
Jan 10, 2020·The Plant Journal : for Cell and Molecular Biology·Fabien Baldacci-CrespSimon Hawkins
Mar 11, 2020·Journal of Experimental Botany·Nerya Zexer, Rivka Elbaum
Dec 29, 2019·The New Phytologist·Yaseen MottiarShawn D Mansfield
Jul 6, 2020·The Plant Journal : for Cell and Molecular Biology·Yasuyuki MiyagawaToshiyuki Takano
Aug 28, 2020·International Journal of Molecular Sciences·Fabien Baldacci-CrespGodfrey Neutelings
Jun 24, 2020·Proceedings of the National Academy of Sciences of the United States of America·Yan ZhuangLei Li
Mar 4, 2020·Nature Plants·Tian XieGanggang Wang
Jul 2, 2019·Trends in Plant Science·Yuanyuan ZhaoJinxing Lin
Nov 4, 2020·Proceedings of the National Academy of Sciences of the United States of America·Nelson Rojas-MurciaNiko Geldner
Oct 23, 2018·Current Opinion in Biotechnology·Mendel PerkinsLacey Samuels
Sep 4, 2020·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Fengmao Li, Yunming Tang
Oct 19, 2021·Frontiers in Plant Science·Leonard Blaschek, Edouard Pesquet
Dec 21, 2021·Frontiers in Plant Science·Lisa S MydyRoland D Kersten
Jan 1, 2022·Plant, Cell & Environment·Salves Cornelis, Ora Hazak

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