Distribution of androgen receptor mRNA expression and immunoreactivity in the brain of the green anole lizard

Journal of Neuroendocrinology
G J RosenJuli S Wade

Abstract

Male courtship and copulation are androgen dependent in the green anole lizard, and female receptivity can be facilitated by testosterone. However, only a few, and relatively large, regions in the brain have been implicated in the control of these behaviours. In situ hybridization and immunohistochemistry were therefore used to determine in detail where androgens are likely to act in the brains of breeding males and females. A 697-bp fragment of the anole androgen receptor (AR) was cloned from total RNA isolated from the kidney, which contains the highly androgen-sensitive renal sex segment. The cloned fragment spanned part of the C, the entire D, and part of the E domains, and shared a high degree of similarity with the AR of various species. 35S-labelled antisense and sense probes were generated from the 697-bp fragment for use in in situ hybridization, and the AR antibody PG-21 was used for immunohistochemistry. Both sexes consistently had AR mRNA expression and immunoreactivity in areas associated with vertebrate reproductive behaviours and in motor areas of the brainstem. Interestingly, the PG-21 antibody produced labelling in both the nucleus and cytoplasm, including neuronal processes. The distribution of mRNA and immuno...Continue Reading

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Citations

Feb 16, 2005·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·Sean L Veney, Juli Wade
Jun 2, 2012·Science·Lauren A O'Connell, Hans A Hofmann
Feb 3, 2016·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Juli Wade
Nov 3, 2009·Physiology & Behavior·Stephany Latham, Juli Wade
May 2, 2008·General and Comparative Endocrinology·Laurel A Beck, Juli Wade
Apr 12, 2008·Hormones and Behavior·Sara SarkeyLydia L DonCarlos
Mar 12, 2008·Brain Research Bulletin·Fernando Martínez-GarcíaEnrique Lanuza
Nov 13, 2007·Physiology & Behavior·David KabelikMichael C Moore
Dec 10, 2008·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Laurel Amanda Beck, Juli Wade
May 22, 2009·Annals of the New York Academy of Sciences·Giulia Guerriero
Jun 25, 2010·The Journal of Comparative Neurology·Lauren A Munchrath, Hans A Hofmann
Nov 29, 2005·Neuroscience·L L DonCarlosL-M Garcia-Segura
Oct 22, 2005·Hormones and Behavior·Juli Wade
May 23, 2002·Hormones and Behavior·Erin L O'Bryant, Juli Wade
Dec 29, 2016·General and Comparative Endocrinology·Alessandra SantilloGabriella Chieffi Baccari
Aug 15, 2003·Journal of Neurobiology·Sean L VeneyJuli Wade
Feb 13, 2021·The Journal of Comparative Neurology·Da-Jiang ZhengSteven M Phelps
Jun 9, 2021·Hormones and Behavior·David KabelikLauren A O'Connell

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