PMID: 40906Jan 1, 1979

Distribution of ATPase in isolated human spermatozoa nuclei: a high resolution cytochemical study

International Journal of Fertility
A SosaA González-Angulo


The presence of ATPase activity was demonstrated in isolated nuclei of human spermatozoa by high resolution cytochemical methods. The Wachstein and Meisell technique as modified by Marchesi and Palade was used. ATPase activity was identified as dense and irregularly distributed granules confined to the exposed surface of spermatozoa nuclei. Within the nucleus the reaction product appeared as electron dense precipitates randomly distributed. Control experiments were negative. Deposits of lead phosphate specifically restricted to the exposed surface of nuclei were interpreted as an indication of a glucose-6-phosphatase and/or phosphohydrolase activity. Whether this activity is located in remnants of the inner leaflet of the nuclear envelope is not known. The presence of the enzyme activity within the nucleus is thought to be related to aerobic ATP synthesis previously suggested. If so, this function may be involved in establishing and/or maintaining the highly complex structural organization of spermatozoa nuclei.

Related Concepts

Adenosine Triphosphatases
ATP Synthesis Pathway
Sperm Cell
Spermatozoa Cell Count
Phosphate Measurement
Cell Nucleus
Nuclear Envelope
Enzyme Activity
Blood Enzyme Activity (Lab Test)

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