Distribution of Brugia malayi larvae and DNA in vector and non-vector mosquitoes: implications for molecular diagnostics.

Parasites & Vectors
Sara M EricksonPeter U Fischer

Abstract

The purpose of this study was to extend prior studies of molecular detection of Brugia malayi DNA in vector (Aedes aegypti- Liverpool) and non-vector (Culex pipiens) mosquitoes at different times after ingestion of infected blood. Parasite DNA was detected over a two week time course in 96% of pooled thoraces of vector mosquitoes. In contrast, parasite DNA was detected in only 24% of thorax pools from non-vectors; parasite DNA was detected in 56% of midgut pools and 47% of abdomen pools from non-vectors. Parasite DNA was detected in vectors in the head immediately after the blood meal and after 14 days. Parasite DNA was also detected in feces and excreta of the vector and non-vector mosquitoes which could potentially confound results obtained with field samples. However, co-housing experiments failed to demonstrate transfer of parasite DNA from infected to non-infected mosquitoes. Parasites were also visualized in mosquito tissues by immunohistololgy using an antibody to the recombinant filarial antigen Bm14. Parasite larvae were detected consistently after mf ingestion in Ae. aegypti- Liverpool. Infectious L3s were seen in the head, thorax and abdomen of vector mosquitoes 14 days after Mf ingestion. In contrast, parasites were...Continue Reading

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Citations

Nov 13, 2010·PLoS Neglected Tropical Diseases·Michelle L MichalskiBruce M Christensen
Aug 15, 2014·PLoS Neglected Tropical Diseases·Mark A SchmaedickKimberly Y Won
Jun 19, 2008·PLoS Neglected Tropical Diseases·Sandra J LaneySteven A Williams
Jun 30, 2009·Trends in Parasitology·Erling M PedersenEdwin Michael
Apr 21, 2016·PLoS Neglected Tropical Diseases·Nils PilotteSteven A Williams
May 20, 2016·Parasitology Research·Kornélia KuruczGábor Kemenesi
Jul 25, 2013·Expert Review of Anti-infective Therapy·Maria P Rebollo, Moses J Bockarie
Apr 9, 2020·PLoS Neglected Tropical Diseases·Corrado MinettiLisa J Reimer
Dec 29, 2020·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Mary M Cameron, Anita Ramesh

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Methods Mentioned

BETA
dissection
PCR
dissections

Software Mentioned

GraphPad Prism

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