Distribution of Cell Envelope Proteinases Genes among Polish Strains of Lactobacillus helveticus

Polish Journal of Microbiology
Katarzyna SkrzypczakAdam Waśko

Abstract

Most of the lactic acid bacteria (LAB) are able to grow in milk mainly due to the activity of a complex and well-developed proteolytic system. Cell envelope-associated proteinases (CEPs) begin casein hydrolysis and allow for releasing the peptides, enclosed in the structure of native milk proteins that are essential for growth of Lactobacillus helveticus. The biodiversity of genes encoding CEPs among L. helveticus strains can have an effect on some technological parameters such as acid production, bacterial growth rate in milk as well as liberation of biologically active peptides. The study reveals significant differences in the presence of various variants of CEPs encoding genes among ten novel Polish strains and indicates the intraspecific diversity exhibited by L. helveticus. In terms of distribution of CEPs genes, four different genetic profiles were found among the microorganisms analyzed. Furthermore, the strains exhibited also various levels of proteolytic activity. Molecular analysis revealed that prtH3 is the most abundant CEPs-encoding gene among the strains investigated. The results indicate also that ecological niche and environmental conditions might affect proteolytic properties of L. helveticus strains. The great...Continue Reading

References

Oct 1, 1996·Antonie van Leeuwenhoek·E R KunjiW N Konings
Apr 22, 2006·Applied Microbiology and Biotechnology·Kirsi SavijokiPekka Varmanen
Mar 1, 2011·International Journal of Food Microbiology·Leila Sadat-MekmeneValérie Gagnaire
Jan 30, 2013·Journal of Dairy Science·L Sadat-MekmeneV Gagnaire

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Datasets Mentioned

BETA
KT285180
KT285181

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

Quantity One
ClustalW
ClustalOmega
Statistica
BLAST

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