Distribution of cell wall hemicelluloses in the wheat grain endosperm: a 3D perspective

Planta
Mathieu FanuelHélène Rogniaux

Abstract

Uneven distribution of AX and BG in lateral and longitudinal dimensions of a wheat grain was observed by three-dimensional MS imaging, presumably related to specific physicochemical properties of cell walls. Arabinoxylans (AX) and β-glucans (BG) are the main hemicelluloses that comprise the primary walls of starchy endosperm. These components are not evenly distributed in the endosperm, and the impact of their distribution on cell wall properties is not yet fully understood. Combined with on-tissue enzymatic degradation of the cell walls, mass spectrometry imaging (MSI) was used to monitor the molecular structure of AX and BG in thirty consecutive cross-sections of a mature wheat grain. A 3D image was built from the planar images, showing the distribution of these polymers at the full-grain level, both in lateral and longitudinal dimensions. BGs were more abundant at the vicinity of the germ and in the central cells of the endosperm, while AX, and especially highly substituted AX, were more abundant close to the brush and in the cells surrounding the crease (i.e., the transfer cells). Compared with the previously reported protocol, significant improvements were made in the tissue preparation to better preserve the shape of the ...Continue Reading

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Citations

Aug 7, 2019·Plant Methods·Thang Duong Quoc LeAnne-Laure Chateigner-Boutin
May 8, 2020·Foods·Yoshimura Yukihiro, Nobuhiro Zaima
Feb 10, 2021·BMC Bioinformatics·Florent GrélardHélène Rogniaux
Mar 30, 2021·Plant Science : an International Journal of Experimental Plant Biology·Anne-Laure Chateigner-BoutinFabienne Guillon
Jul 17, 2019·Journal of Agricultural and Food Chemistry·Zhiqiang ShiMalcolm J Hawkesford

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Methods Mentioned

BETA
fluorescence microscopy

Software Mentioned

SCiLS Lab
Flex Control
Flex Imaging
SCiLS

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