Distribution of cepacian biosynthesis genes among environmental and clinical Burkholderia strains and role of cepacian exopolysaccharide in resistance to stress conditions.

Applied and Environmental Microbiology
Ana S FerreiraLeonilde M Moreira

Abstract

The genus Burkholderia includes strains pathogenic to animals and plants, bioremediators, or plant growth promoters. Genome sequence analyses of representative Burkholderia cepacia complex (Bcc) and non-Bcc strains for the presence of the bce-I gene cluster, directing the biosynthesis of the exopolysaccharide (EPS) cepacian, further extended this previously described cluster by another 9 genes. The genes in the bce-II cluster were named bceM to bceU and encode products putatively involved in nucleotide sugar precursor biosynthesis and repeat unit assembly, modification, and translocation across the cytoplasmic membrane. Disruption of the B. cepacia IST408 bceQ and bceR genes, encoding a putative repeat unit flippase and a glycosyltransferase, respectively, resulted in the abolishment of cepacian biosynthesis. A mutation in the bceS gene, encoding a putative acyltransferase, did not affect EPS production yield significantly but decreased its acetylation content by approximately 20%. Quantitative real-time reverse transcription-PCR experiments confirmed the induction of genes in the bce-I and bce-II clusters in a Burkholderia multivorans EPS producer clinical isolate in comparison to the level for its isogenic EPS-defective strai...Continue Reading

References

May 1, 1987·Applied and Environmental Microbiology·M J NelsonP H Pritchard
May 1, 1966·Journal of General Microbiology·R Y StanierM Doudoroff
Apr 24, 1993·Lancet·C Koch, N Høiby
Dec 1, 1994·Microbiological Reviews·M Potts
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Jul 13, 2000·Biochemical and Biophysical Research Communications·P CescuttiI Sá-Correia
Sep 28, 2000·Biochemical and Biophysical Research Communications·J A RichauI Sá-Correia
Apr 26, 2001·International Journal of Systematic and Evolutionary Microbiology·T CoenyeP Vandamme
Aug 9, 2001·International Journal of Systematic and Evolutionary Microbiology·T CoenyeP Vandamme
Feb 14, 2003·Systematic and Applied Microbiology·Peter VandammeAnne Willems
Nov 26, 2003·Biochemical and Biophysical Research Communications·Leonilde M MoreiraIsabel Sá-Correia
Sep 25, 2004·International Journal of Systematic and Evolutionary Microbiology·Johan GorisPeter Vandamme
Oct 7, 2004·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Luigi ChiariniRoberto Rizzo
Jan 12, 2005·Nature Reviews. Microbiology·Eshwar MahenthiralingamJoanna B Goldberg
May 10, 2005·International Journal of Systematic and Evolutionary Microbiology·A SessitschJ Nowak
Jul 25, 2006·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Yury HerasimenkaRoberto Rizzo
Dec 23, 2006·Biochemical and Biophysical Research Communications·Sílvia A SousaJorge H Leitão
Jul 14, 2007·Cellular Microbiology·Silvia A SousaGerd Döring
Oct 16, 2007·Nucleic Acids Research·Victor M MarkowitzNikos C Kyrpides
Feb 8, 2008·Journal of Clinical Microbiology·James E A ZlosnikDavid P Speert
Nov 6, 2008·Annual Review of Genetics·Katherine E GibsonGraham C Walker
Jan 1, 1995·Annual Review of Phytopathology·T P Denny

❮ Previous
Next ❯

Citations

May 21, 2013·Applied and Environmental Microbiology·Bruna G CoutinhoVittorio Venturi
Sep 12, 2013·PloS One·Gabriella PessiLeo Eberl
Aug 19, 2011·Microbial Ecology·Zulma Rocío Suárez-MorenoVittorio Venturi
Aug 13, 2013·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Anne-Sophie MessiaenTom Coenye
Dec 20, 2015·Future Microbiology·Joana R FelicianoJorge H Leitão
Jun 2, 2015·Frontiers in Microbiology·Gregory B WhitfieldP Lynne Howell
Jun 4, 2015·PloS One·Yi-Huang HsuehChao-Lung Chiang
Oct 29, 2013·Journal of Bacteriology·Jennifer A BartellJason A Papin
May 13, 2014·Applied and Environmental Microbiology·Jiyeun Kate KimBok Luel Lee
Jan 28, 2014·Journal of Bacteriology·Charlotte MajerczykE Peter Greenberg
Apr 5, 2015·Applied and Environmental Microbiology·Martina LardiGabriella Pessi
Apr 15, 2010·Applied Microbiology and Biotechnology·Jorge H LeitãoLeonilde M Moreira
Mar 1, 2017·Proceedings of the National Academy of Sciences of the United States of America·Lindsey S MarmontP Lynne Howell
Dec 6, 2017·Microbial Biotechnology·Teresa BerningerAngela Sessitsch
May 2, 2021·Applied and Environmental Microbiology·Sara C GomesLeonilde M Moreira
Aug 11, 2021·MBio·Matthew M SchaefersGregory P Priebe

❮ Previous
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