PMID: 6989839May 1, 1980Paper

Distribution of chitin in the yeast cell wall. An ultrastructural and chemical study

The Journal of Cell Biology
J MolanoE Cabib

Abstract

The distribution of chitin in Saccharomyces cervisiae primary septa and cell walls was studied with three methods: electron microscopy of colloidal gold particles coated either with wheat germ agglutinin or with one of two different chitinases, fluorescence microscopy with fluorescein isothiocyanate derivatives of the same markers, and enzymatic treatments of [14C]glucosamine-labeled cells. The septa were uniformly and heavily labeled with the gold-attached markers, an indication that chitin was evenly distributed throughout. To study the localization of chitin in lateral walls, alkali-extracted cell ghosts were used. Observations by electron and fluorescence microscopy suggest that lectin-binding material is uniformly distributed over the whole cell ghost wall. This material also appears to be chitin, on the basis of the analysis of the products obtained after treatment of 14C-labeled cell ghosts with lytic enzymes. The chitin of lateral walls can be specifically removed by treatment with beta-(1 leads to 6)-glucanase containing a slight amount of chitinase. During this incubation approximately 7% of the total radioactivity is solubilized, about the same amount liberated when lateral walls of cell ghosts are completely digeste...Continue Reading

References

Oct 24, 1977·Archives of Microbiology·M Horisberger, M Vonlanthen
Dec 1, 1977·Analytical Biochemistry·J MolanoE Cabib
Oct 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·A DuránE Cabib
Jan 1, 1972·Archiv für Mikrobiologie·H BauerE Sigarlakie
Sep 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·E Cabib, V Farkas
Sep 15, 1960·Experientia·H RINDERKNECHT

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Citations

Aug 8, 2002·FEMS Microbiology Reviews·Frans M KlisStanley Brul
Sep 1, 1986·Journal of Helminthology·P DubinskýE Ossikovski
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·E CabibR L Roberts
May 1, 1989·The Journal of Cell Biology·E CabibS J Silverman
Jul 1, 1991·The Journal of Cell Biology·J A ShawE Cabib
Nov 26, 2004·Molecular Biology of the Cell·Quan ZhongMiriam L Greenberg
May 5, 2006·Molecular Biology of the Cell·Gertien J SmitsFrans M Klis
Jul 15, 1991·European Journal of Biochemistry·A R ButlerM J Stark
Jan 1, 1995·Antimicrobial Agents and Chemotherapy·M el-Sherbeini, J A Clemas
Sep 6, 2008·Microbiology and Molecular Biology Reviews : MMBR·W LaJean Chaffin
Jan 1, 1985·Critical Reviews in Microbiology·D PoulainA Vernes
Jan 1, 1987·Critical Reviews in Microbiology·M G Shepherd
Jan 8, 2014·Expert Opinion on Therapeutic Patents·María Victoria CastelliSusana A Zacchino
Jan 1, 1991·Critical Reviews in Biotechnology·S Bielecki, E Galas
Jul 1, 1994·Yeast·F M Klis
Jul 20, 2010·Yeast·Katja BackhausJürgen J Heinisch
Jun 1, 1981·Biochemical and Biophysical Research Communications·M C BertheR Bonaly
Jun 29, 2005·Journal of Biotechnology·Stefan RyckaertRoland Contreras
Jan 15, 1991·Biochemical and Biophysical Research Communications·S J SilvermanE Cabib
Jul 16, 2014·Journal of Biotechnology·Franziska BühligenSusann Müller
Oct 30, 2016·Biochimie·Marina RautenbachJ Arnold Vosloo
Feb 13, 2001·Microscopy Research and Technique·M MolinaC Nombela
Jan 15, 2002·Yeast·Akalpita U Arvindekar, Narayan B Patil
Jul 12, 2002·Yeast·James A Barnett, Carl F Robinow
Aug 21, 2013·Nature Reviews. Microbiology·Enrico Cabib, Javier Arroyo
Jan 20, 1995·The Journal of Biological Chemistry·R KollárE Cabib
Aug 1, 1981·Journal of Neurochemistry·R M CawthonX O Breakefield
Jan 1, 1982·Zeitschrift für allgemeine Mikrobiologie·J NüskeG Reuter
Jan 1, 1985·Radiation and Environmental Biophysics·C Baumstark-KhanA Wegener

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