Distribution of components of the SNARE complex in relation to transmitter release sites at the frog neuromuscular junction

The European Journal of Neuroscience
J A BoudierM J Seagar

Abstract

At the frog neuromuscular junction, neurotransmitter release sites are regularly spaced at 1 micron intervals along the nerve terminal, directly facing postsynaptic folds which contain a high density of acetylcholine receptors. Immunostaining and laser confocal scanning microscopy were used to compare the distribution of presynaptic proteins implicated in exocytosis with that of fluorescent alpha-bungarotoxin. Syntaxin, synaptosome-associated 25 kDa protein and calcium channels were located predominantly at release sites. Synaptobrevin (vesicle-associated membrane protein) was distributed in the cytoplasm of the nerve terminal, presumably in the packets of microvesicles associated with each active zone. N-Ethylmaleimide-sensitive fusion protein (NSF) and soluble NSF attachment proteins (alpha beta SNAP) displayed a diffuse distribution throughout the terminal cytoplasm and also colocalized in distinct concentrated zones adjacent to the presynaptic membrane.

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Citations

Sep 29, 2001·Traffic·A LinM Latterich
Oct 10, 1998·The European Journal of Neuroscience·D A RaciborskaM P Charlton
Apr 23, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·M SeagarM Takahashi
Oct 19, 2000·The Journal of Cell Biology·E GroteP J Novick
Jul 29, 1998·General and Comparative Endocrinology·J L QuintanarJ A Reig
Aug 26, 1998·Biochimica Et Biophysica Acta·B J Nichols, H R Pelham
Nov 17, 1998·Microscopy Research and Technique·M R BennettK L Brain
Sep 1, 2017·Traffic·Tuanlao WangWanjin Hong
Jun 23, 1998·The Journal of Biological Chemistry·K G FlemingP I Hanson

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