Distribution of de novo Donor-Specific Antibody Subclasses Quantified by Mass Spectrometry: High IgG3 Proportion Is Associated With Antibody-Mediated Rejection Occurrence and Severity.

Frontiers in Immunology
Vincent PerninMoglie Le Quintrec

Abstract

Donor-specific antibodies (DSAs) are the main risk factor for antibody-mediated rejection (ABMR) and graft loss but could have variable pathogenicity according to their IgG subclass composition. Luminex-based test might lack sensitivity for the detection of IgG subclasses and this test does not allow quantifying the relative abundance of each IgG subclass. We investigated the precise repartition of each DSA subclass and their role in ABMR occurrence and severity, using an innovative mass spectrometry-based method. Between 2014 and 2018, we enrolled 69 patients who developed de novo DSA (n = 29 without ABMR, and n = 40 with ABMR) in two transplant centers. All IgG subclasses were detected in every samples tested: 62.7% were IgG1, 26.6% were IgG2, 6.6% were IgG3, and 4.2% were IgG4. The IgG3 proportion was significantly higher in the ABMR+ compared to the ABMR- group (8.4% vs. 5.6%, p = 0.003). The proportion of IgG1, IgG2, and IgG4 of DSA was similar between the two groups. Higher IgG3 level was associated with higher C4d deposition, higher microvascular inflammation scores, and glomerular filtration rate decline >25%. IgG3 proportion was not correlated with DSA MFI. Multivariate analysis showed that proteinuria and high level o...Continue Reading

References

Jan 1, 1993·Advances in Immunology·R L CoffmanP Rothman
Jul 22, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Danielle T AveryStuart G Tangye
Jul 17, 2010·Journal of the American Society of Nephrology : JASN·Carmen LefaucheurCaroline Suberbielle-Boissel
Nov 16, 2011·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·J SellarésP F Halloran
Mar 21, 2012·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·C WiebeP W Nickerson
Apr 18, 2012·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Hugo KanekuGöran B Klintmalm
Oct 3, 2012·Nature Reviews. Nephrology·Mark D StegallLynn D Cornell
Feb 6, 2013·Transplantation·Matthew J EverlyPaul I Terasaki
Sep 27, 2013·The New England Journal of Medicine·Alexandre LoupyXavier Jouven
Feb 4, 2014·Transplantation·Matthew J EverlyPaul I Terasaki
Mar 17, 2015·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·J G O'LearyP I Terasaki
Aug 13, 2015·Transplant International : Official Journal of the European Society for Organ Transplantation·Natasha KhovanovaDavid Lowe
Aug 22, 2015·Journal of the American Society of Nephrology : JASN·Carmen LefaucheurAdriana Zeevi
Dec 16, 2016·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·C A SchinstockM D Stegall
Mar 4, 2017·Journal of the American Society of Nephrology : JASN·Olivier AubertPhilip F Halloran
Dec 16, 2017·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·M HaasM Mengel
Dec 20, 2017·Journal of the American Society of Nephrology : JASN·Denis VigliettiCarmen Lefaucheur
Apr 20, 2018·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Maxime CourantJean-Luc Taupin
Jun 1, 2018·Journal of Transplantation·Maria Cristina Ribeiro de CastroElias David-Neto
Jun 2, 2018·Pediatric Transplantation·Gilad HamdaniAlin L Girnita

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Methods Mentioned

BETA
biopsy
flow cytometry

Clinical Trials Mentioned

NCT04026087

Software Mentioned

Analyst TF
STATA
GraphPad Prism
ProteinPilot
GraphPad

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