PMID: 15227801Jul 2, 2004Paper

Distribution of E and NS1 proteins of TBE virus in mammalian and tick cells

Folia Microbiologica
F SeniglL Grubhoffer

Abstract

Four monoclonal antibodies recognizing TBEV proteins were prepared. Three of them (2/H6, 8/A10, 5/F9) specifically recognize the structural E protein and the fourth binds to the nonstructural NS1 protein. These antibodies were used for an immunofluorescence study of TBEV protein distribution in infected mammalian host and tick vector tissue culture cells. Any differences in the distribution of the E and NS1 proteins were revealed. In porcine PS cell line the proteins were localized in the cytoplasm with a distinct increase of the signal in the perinuclear area 16 h post-infection (p.i.). The area gradually expanded and at 24 h p.i. covered the major part of the cytoplasm. The localization of the proteins in tick RA-257 cells revealed a uniform spread of the proteins in whole cytoplasm of the infected cells. The signal was very weak at 16 h p.i. and gradually increased during progressing time p.i., although, the distribution of the proteins did not exhibit any changes. Difference in viral protein distribution in the two cell types points to the possibility that the maturation process of TBEV exhibits different features in mammalian and tick cells.

References

Jan 1, 1990·Annual Review of Microbiology·T J ChambersC M Rice
Jan 1, 1987·Advances in Virus Research·E G Westaway

❮ Previous
Next ❯

Citations

Oct 1, 2011·Vector Borne and Zoonotic Diseases·Lesley Bell-SakyiJohn K Fazakerley
Jul 31, 2007·Trends in Parasitology·Lesley Bell-SakyiFrans Jongejan
Jul 4, 2019·Critical Reviews in Microbiology·Aurélie VelaySamira Fafi-Kremer
Jul 1, 2021·Pathogens and Global Health·Cristiano SalataLesley Bell-Sakyi
Nov 28, 2021·Viruses·Zdenek Hubálek

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.