Nov 9, 2018

Distribution of endogenous normal tau in the mouse brain

The Journal of Comparative Neurology
Atsuko KuboTomohiro Miyasaka

Abstract

Tau is a microtubule-associated protein (MAP) that is localized to the axon. In Alzheimer's disease (AD), the distribution of tau undergoes a remarkable alteration, leading to the formation of tau inclusions in the somatodendritic compartment. While the abnormal aggregated tau has been extensively studied in human patient tissues and animal models of AD, how normal tau localizes to the axon, which would be the foundation to understand how the mis-localization occurs, has not been well studied due to the poor detectability of normal unaggregated tau in vivo. Therefore, we developed immunohistochemical techniques that can detect normal mouse and human tau in brain tissues with high sensitivity. Using these techniques, we demonstrate the global distribution of tau in the mouse brain and confirmed that normal tau is exclusively localized to the axonal compartment in vivo. Interestingly, tau antibodies strongly labeled nonmyelinated axons such as hippocampal mossy fibers, while white matters generally exhibited low levels of immunoreactivity. Furthermore, mouse tau is highly expressed not only in neurons but also in oligodendrocytes. With super resolution imaging using the stimulated-depletion microscopy, axonal tau appeared punctat...Continue Reading

  • References35
  • Citations7

References

  • References35
  • Citations7

Citations

Mentioned in this Paper

Biological Markers
Establishment and Maintenance of Localization
In Vivo
Presynaptic Terminals
Co-treatment
Microtubule-Associated Proteins
tau-1 monoclonal antibody
Immunohistochemistry
Mossy Fibers, Hippocampal
Immunoreactivity

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