Aug 10, 1976

Distribution of enzyme activities in subcellular fractions of bovine retina

The Journal of Biological Chemistry
W F ZimmermanS L Bonting

Abstract

Centrifugation of homogenates of bovine retinas to isopycnic equilibrium in sucrose density gradients yielded three partially overlapping bands of particles which were, in the order of increasing density: (a) photoreceptor cell (rod) outer segments; (b) plasma membranes, lysosomes, and large fragments of endoplasmic reticulum; and (c) mitochondria. The only enzyme activity investigated which had a peak coinciding only with outer segment fractions was guanylate cyclase. Enzyme activities with peaks in both the outer segment and denser fractions included 5'-nucleotidase and cyclic GMP phosphodiesterase. Enzyme activities with peaks only in the denser fractions included sodium and potassium ion-activated ATPase ((Na+ + K+)-ATPase), adenylate cyclase, cyclic AMP phosphodiesterase, beta-glucosidase, beta-galactosidase, and succinate-dependent cytochrome c reductase. These results suggest that some of the activities once thought to be present in rod outer segments are actually present in particles from elsewhere in the retina which contaminate rod outer segment preparations.

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Mentioned in this Paper

Photoreceptors
Nucleotidases
Glucosephosphatase
Rhodopsin
Entire Retina
Succinate Cytochrome c Oxidoreductase
Adenosine Triphosphatases
Cyclic GMP Measurement (Lab Test)
Phosphoric diester hydrolase
Succinates

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