Abstract
The distribution of free and HLA-associated human beta 2-microglobulin (beta 2m) in serum, urine, spinal fluid, parotid duct saliva, seminal fluid, amniotic fluid and whey and in membranes from thrombocytes, lymphocytes, neutrophils and fat globules from milk was studied by crossed radioimmunoelectrophoresis (CRIE). The hydrophobic domain of HLA was demonstrable in 'charge-shift CRIE' and by binding to phenyl-Sepharose in 'hydrophobic-interaction CRIE'. In 'lectin-affinity CRIE' with concanavalin A and Lens culinaris lectin Sepharose the carbohydrate moiety present on. HLA exhibited heterogeneity as judged by the appearance of two partly separated protein peaks. Except for isolated fat globule membranes, HLA-associated beta 2m was present on all cells investigated. 'Free' beta 2m did not contain a hydrophobic domain as assessed by charge-shift and hydrophobic-interaction CRIE. All body fluids contained beta 2m in its 'free' form only. In serum, besides the free beta 2m, 2% was present as HLA-associated beta 2m, which, however, did not contain a hydrophobic domain. A degradation product of 'free' beta 2m, with alpha-mobility, was observed in sera from patients with malignant disorders, rheumatoid arthritis or systemic lupus eryt...Continue Reading
References
Jun 5, 1978·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·M J CrumptonW F Bodmer
Dec 1, 1975·Scandinavian Journal of Clinical and Laboratory Investigation·T PlesnerT Boenisch
Jan 1, 1977·Advances in Cancer Research·P A PetersonL Ostberg
Jan 1, 1978·Scandinavian Journal of Immunology·T Plesner
Mar 1, 1979·Scandinavian Journal of Immunology·T Plesner, A Wiik
May 2, 1977·Biochimica Et Biophysica Acta·C S Nielsen, O J Bjerrum
Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A Helenius, K Simons
Aug 15, 1979·European Journal of Biochemistry·I HagenN O Solum
Oct 1, 1978·Analytical Biochemistry·O J Bjerrum
Oct 3, 1977·Biochimica Et Biophysica Acta·S BhakdiO J Bjerrum
Nov 20, 1975·Nature·D SnaryM J Crumpton
Mar 1, 1973·Transplantation·Y MiyakawaD Pressman
Jan 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·P A PetersonJ B Lindblom
Feb 15, 1974·Nature·D SnaryM J Crumpton
Jan 1, 1973·Annual Review of Biochemistry·H Lis, N Sharon
Sep 1, 1965·The Biochemical Journal·P Andrews
Citations
Feb 28, 1986·Clinica Chimica Acta; International Journal of Clinical Chemistry·O W Bjerrum, H S Birgens
Jan 1, 1987·Leukemia Research·O W Bjerrum, P Gimsing
Jun 1, 1982·Molecular Immunology·E Lillehoj, M D Poulik
Jul 1, 1993·Tissue Antigens·P PoulettyH Grosse-Wilde
Jan 1, 1994·Vox Sanguinis·K HillebrandH Grosse-Wilde
Nov 1, 1989·Blut·I DoxiadisH Grosse-Wilde
Jan 1, 1995·Ocular Immunology and Inflammation·A A OkadaM Usui
Apr 1, 1996·Leukemia & Lymphoma·K RemesT T Pelliniemi
Sep 1, 1990·Scandinavian Journal of Immunology·O W BjerrumN Borregaard
Jun 1, 1993·Kidney International·P Zaoui, R M Hakim
Nov 1, 1990·Kidney International·P M ZaouiR M Hakim
Sep 1, 1993·Kidney International·R M Hakim
Mar 12, 2005·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Stefan SchaubPeter Nickerson
Feb 16, 1987·European Journal of Biochemistry·M H NissenM Christensen
Feb 1, 1982·Journal of Dairy Science·M L Groves, R Greenberg
Sep 1, 1986·Acta Neurologica Scandinavica·O W BjerrumO E Hansen
Jan 1, 1989·Vox Sanguinis·C M GilesM Botto
Dec 12, 2007·Scandinavian Journal of Immunology·M WangM H Nissen
Dec 1, 1983·Archives of Andrology·J LizanaP Eneroth
Jul 14, 2011·Proteomics. Clinical Applications·Olwyn JohnstonTara McMorrow
May 9, 2015·Proteomics. Clinical Applications·Hilary CassidyTara McMorrow
Jan 1, 1981·Tissue Antigens·W F Bodmer
Jul 1, 1985·Scandinavian Journal of Haematology·O W Bjerrum, T Plesner
Oct 1, 1985·Scandinavian Journal of Haematology·S AvnstrømN I Nissen
Oct 1, 1982·Tissue Antigens·M E DehP Donnai
Feb 4, 1982·Biochimica Et Biophysica Acta·I HangenN O Solum
Nov 1, 1984·Infection and Immunity·D Ericson
Jun 10, 2000·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·R DeicherW H Hörl
Mar 19, 2003·Analytical Chemistry·Jette W SenNiels H H Heegaard