PMID: 2508624Aug 1, 1989Paper

Distribution of G-proteins in rat liver plasma-membrane domains and endocytic pathways

The Biochemical Journal
N AliW H Evans

Abstract

1. The distribution of the alpha- and beta-subunits of nucleotide-binding G-proteins among rat liver sinusoidal, lateral and canalicular plasma membranes, endosomes, Golgi membranes and lysosomes was investigated. 2. Pertussis-toxin-catalysed ADP-ribosylation identified a 41 kDa inhibitory alpha-subunit in all liver plasma-membrane functional domains as well as in endosomes. An antibody to a synthetic peptide corresponding to a C-terminal sequence of the inhibitory alpha-subunit also identified the 41 kDa polypeptide in all plasma-membrane domains, in 'early' and 'late' endosomes and in Golgi membranes; this polypeptide was not detected in lysosomes. The antibody-binding studies showed that bile-canalicular plasma membranes had the highest content of the inhibitory alpha-subunit. 3. Immunofluorescent microscopy confirmed the presence of the inhibitory alpha-subunit in all regions of the hepatocyte's cell surface. 4. An antibody recognizing the beta-subunit showed that a 36 kDa polypeptide was present in all plasma membranes and in 'early' and 'late' endosomes; it was not detected in lysosomes. The relative distribution among the fractions of this polypeptide was similar to the distribution of the inhibitory alpha-subunit. 5. Th...Continue Reading

Citations

Dec 31, 1997·Microscopy Research and Technique·W J Gallin
Sep 1, 1996·Journal of Cellular Biochemistry·M CadrinN Bégin-Heick
Nov 14, 1997·The Journal of Cell Biology·D M WardJ Kaplan
Dec 1, 1994·Journal of Pharmacological and Toxicological Methods·N Ali, D K Agrawal
Apr 1, 1992·Trends in Cell Biology·F A BarrW B Huttner
Mar 1, 1994·Acta histochemica·W SchulzeL Will-Shahab
Nov 1, 1996·Acta histochemica·L W DidenkoE Unger

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