PMID: 3772373Dec 1, 1986Paper

Distribution of glial fibrillary acidic protein in gliosed human white matter

Journal of Neurochemistry
J NewcombeM L Cuzner

Abstract

Glial fibrillary acidic protein (GFAP) in gliosed white matter from multiple sclerosis plaques and cerebral infarcts was examined by polyacrylamide gel electrophoresis and immunoblotting. Using a monoclonal antibody raised against human GFAP, up to 11 GFAP polypeptide bands of molecular weight 37-49 kilodaltons were identified in particulate and supernatant fractions of CNS tissue homogenates. Soluble GFAP constituted about one-quarter of the total GFAP in normal cerebral white matter. In brain lesions in which reactive astrocytes were observed microscopically, the proportion of soluble GFAP was increased, with a greater representation of the lower-molecular-weight forms. In brain chronic sclerotic plaques, almost all of the GFAP was in the particulate form. Purified particulate GFAP was susceptible to proteolysis at acid but not at neutral pH in the presence of CNS homogenates. In tissue autolysis studies, GFAP was stable in situ for periods well in excess of average CNS postmortem times.

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Citations

Aug 1, 1987·Journal of the Neurological Sciences·G M HayesM L Cuzner
Aug 1, 1990·Journal of the Neurological Sciences·O S JørgensenR Balázs
Jul 1, 1995·Journal of the Neurological Sciences·C T Bever, D W Garver
Jun 28, 2005·Experimental Eye Research·Akiyasu KanamoriAkira Negi
May 14, 2003·Journal of Neuroimmunology·A PetzoldG Giovannoni
Jul 27, 1999·Neuropathology and Applied Neurobiology·D GvericJ Newcombe
Apr 1, 1994·Neuropathology and Applied Neurobiology·J NewcombeM L Cuzner
Jul 1, 1996·Brain Pathology·M L Cuzner, W T Norton
Nov 28, 2001·Journal of Neurology, Neurosurgery, and Psychiatry·M Siger-Zajdel, K Selmaj
Jan 23, 2013·Brain Research·Marco WeberHeike Franke
Jun 4, 2008·Journal of Neuropathology and Experimental Neurology·Peter J DarlingtonJack P Antel
Mar 11, 2009·Neuroscience·S NagarN K Dhingra
Oct 11, 2007·Brain Pathology·Jia NewcombeTerence Smith
Jan 30, 2015·Acta Neuropathologica Communications·Chiara NicoliniMargaret Fahnestock
Nov 1, 1988·Neuropathology and Applied Neurobiology·J NewcombeM L Cuzner
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Sep 26, 2003·Neuropathology and Applied Neurobiology·J E HolleyN J Gutowski
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Feb 29, 2020·Cells·Stéphane Mélik ParsadaniantzChristophe Baudouin
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Sep 6, 2019·The Journal of Biological Chemistry·Michael R HeavenMichael Brenner
Jul 28, 2017·Research in Veterinary Science·A R Intan-ShamehaHussni O Mohammed

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