Distribution of Goodpasture antigens within various human basement membranes

Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association
M WeberH Köhler

Abstract

Collagenase-digested basement-membrane preparations from human kidney glomeruli, kidney tubules, lung, choroid plexus, aorta, intestine, and placenta were analysed according to their reactivity to anti-glomerular basement membrane (anti-GBM) antibody-positive Goodpasture sera. Sodium dodecylsulphate polyacryl gel electrophoresis (SDS-PAGE), and immunoblotting were performed after antigen enrichment by passage of the collagenase digests through an anion-exchange column. Reactivity of anti-GBM antibodies with one to three monomers (24, 26 and 28 kD) and two dimers (44 and 50 kD) were demonstrated in basement membrane preparations of kidney glomeruli, kidney tubules, lung, placenta, and aorta. In basement membranes of choroid plexus reactivity with only the 28 kD monomer and the 50 kD dimer were identified. In intestinal basement membrane, reactivity was restricted to the 50 kD dimer. Analysis of the amounts of Goodpasture antigen by inhibition ELISA demonstrated that the highest concentration were in glomerular basement membrane, while the lowest were found in aortic basement membrane. The results indicate that Goodpasture antigens are common to all the basement membranes investigated. The differences in antigen concentration and...Continue Reading

Citations

Apr 23, 2002·Journal of Clinical Laboratory Analysis·Troy D JaskowskiHarry R Hill
May 7, 2004·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Ernesto PaolettiGiuseppe Cannella
Oct 7, 2010·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Julia KolbPeter Kotanko
Jan 10, 2001·Current Opinion in Rheumatology·U Specks
Sep 1, 1992·Journal of Clinical Pathology·J M SerotG C Faure
Aug 1, 1991·American Journal of Respiratory Cell and Molecular Biology·E C Crouch
Feb 1, 1995·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·H DeubnerC E Alpers
Feb 1, 2014·Clinical Kidney Journal·Masafumi SugiyamaMasanori Funauchi
Apr 25, 2021·Journal of the American Society of Nephrology : JASN·Cong-Rong ShenDorin-Bogdan Borza
Mar 23, 2002·The American Journal of the Medical Sciences·Schlomo Cohen

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