Distribution of heparin-binding EGF-like growth factor protein and mRNA in the normal rat kidneys

Kidney International
T NakagawaF Hazama

Abstract

Heparin-binding EGF-like growth factor (HB-EGF), a newly discovered potent mitogen and chemoattractant for smooth muscle cells, is a member of the EGF superfamily and binds to EGF receptors. To investigate the role of HB-EGF in the kidney, we determined the distribution of HB-EGF immunohistochemically in normal rat kidneys. The localization of mRNA expression was also studied by in situ hybridization, using a synthesized digoxigenin-labeled anti-sense riboprobe of HB-EGF. Immunohistochemical and in situ hybridization studies revealed that the tubular epithelial cells of the S3 segment of the outer stripe in the outer medulla were the predominant renal source of HB-EGF. In addition, in the immunohistochemical analysis, HB-EGF was ubiquitously present in the epithelial cells of the proximal tubules and the arterial smooth muscle cells, while HB-EGF expression was not detected in other parts of the kidney, including the glomeruli. Although EGF receptors were found to be present in the proximal tubules as well as in the distal tubules and collecting ducts, EGF has not been found to be expressed in the proximal tubules. Therefore, the present results indicate that HB-EGF might be a ligand for EGF receptors in the proximal tubules an...Continue Reading

References

Feb 1, 1995·Kidney International·S A OrellanaE D Avner
Dec 6, 1994·Proceedings of the National Academy of Sciences of the United States of America·D Aviezer, A Yayon

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Citations

Apr 6, 2004·Molecular and Cellular Endocrinology·Bochen PanMutsuo Ishikawa
Aug 26, 2000·Cytokine & Growth Factor Reviews·R Iwamoto, E Mekada
Apr 3, 2012·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Martin FlamantPierre-Louis Tharaux
Mar 17, 1999·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·N KawaharaT Kirino
May 2, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jian-Kang ChenRaymond C Harris
Feb 19, 2016·Drug and Chemical Toxicology·Peter Luke Santa MariaYunzhi Peter Yang
Aug 28, 2015·Kidney International Supplements·Shougang Zhuang, Na Liu
Jul 5, 2016·Nature Reviews. Nephrology·Laura R HarskampEsther Meijer
Jan 26, 2018·The Journal of Clinical Investigation·Xiaoping QingJane E Salmon
Apr 13, 2004·American Journal of Physiology. Renal Physiology·Shougang ZhuangShougang Zhougang
May 3, 2013·American Journal of Physiology. Renal Physiology·Alexander StaruschenkoTengis S Pavlov
Dec 1, 2017·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·Daniel M BeswickPeter Luke Santa Maria
Jul 1, 2016·Physiological Reviews·Jianchun ChenRaymond C Harris
Apr 7, 2000·Journal of the American Society of Nephrology : JASN·Rajeev M MahimkarDavid H Lovett
Nov 7, 1999·The American Journal of Pathology·T NakagawaF Hazama
Jun 24, 2003·The American Journal of Pathology·Mikiko Takikita-SuzukiRyuichi Kikkawa

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