Distribution of human colonic dendritic cells and macrophages

Clinical and Experimental Immunology
P PavliF Doe

Abstract

To define the phenotype of intestinal dendritic cells and macrophages, resected colonic specimens were used to obtain lamina propria cell suspensions by EDTA treatment, then enzymatic digestion. The phenotype of dendritic cell-enriched suspensions was compared with that of macrophage-enriched populations by immunocytochemistry using the avidin-biotin-peroxidase (ABC) system and immunoelectron microscopy. Dendritic cells expressed HLA-DR (L243) and HLA-DQ-associated (RFD) antigens and CD68 in a perinuclear distribution. Staining for S100 was weak or absent. Macrophages also expressed HLA markers (L243 and RFD1) and CD68. The 25F9 antigen was expressed strongly, whilst CD14 was absent from cells isolated from noninflamed tissues. To determine their anatomic distribution, immunohistochemistry was performed using single- and double-labelling techniques (ABC +or- alkaline phosphatase anti-alkaline phosphatase method). Mutually exclusive subsets of 25F9+ and S100+ cells were seen: 25F9+ macrophages were concentrated in a band immediately beneath the luminal epithelium; S100+/HLA-DR+ dendritic cells formed a reticular network throughout the lamina propria and beneath the basement membrane of the crypts. This distribution suggests that...Continue Reading

Citations

Dec 19, 2000·Scandinavian Journal of Immunology·M UlanovaE Telemo
Feb 10, 2006·Clinical and Vaccine Immunology : CVI·Benoît DesnuesJean-Louis Mege
Mar 24, 2007·Annual Review of Immunology·Akiko Iwasaki
May 10, 2005·American Journal of Respiratory and Critical Care Medicine·Karim Vermaelen, Romain Pauwels
Mar 13, 2013·Trends in Immunology·Ehud Zigmond, Steffen Jung
Mar 19, 2014·Journal of Biomedical Science·Leh-Miauh Buu, Yee-Chun Chen
Jan 26, 2000·The Laryngoscope·G M SprinzlA Schrott-Fischer
Jun 27, 1998·Leukemia & Lymphoma·S P RobinsonC D Reid
Feb 1, 2005·Annals of the New York Academy of Sciences·Gordon MacPhersonFang-Ping Huang
Mar 20, 2004·Clinical and Experimental Immunology·M HausmannG Rogler
Sep 13, 2003·Parasite Immunology·Thomas T MacDonald
Apr 16, 2008·Journal of Gastroenterology and Hepatology·Anna B PulimoodKunnissery A Balasubramanian
Jan 29, 2000·Japanese Circulation Journal·H YokoyamaT Izumi
Jun 28, 2005·Seminars in Immunology·Cecilia Johansson, Brian L Kelsall
May 29, 1998·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·M JontellG Bergenholtz
Oct 9, 2004·Inflammatory Bowel Diseases·Manuel A SilvaErnest G Seidman
Sep 30, 2008·Inflammatory Bowel Diseases·Manuel A Silva
Aug 1, 2012·International Archives of Allergy and Immunology·Carolin PilznerDavid A Groneberg
May 26, 2010·Nature Reviews. Immunology·Chen VarolSteffen Jung
Nov 8, 2017·BMC Veterinary Research·Yao LeeGisela Soboll Hussey
Jan 27, 2004·The Journal of Pathology·Frédérique LarousserieOdile Devergne
May 8, 2000·Clinical Anatomy : Official Journal of the American Association of Clinical Anatomists & the British Association of Clinical Anatomists·S AbrahamG Chandi
Apr 6, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·S J BellA J Stagg
Jul 2, 2004·Experimental Biology and Medicine·Mark M Huycke, H Rex Gaskins
Jul 22, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Martin HausmannGerhard Rogler

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.