Distribution of insecticide resistance and mechanisms involved in the arbovirus vector Aedes aegypti in Laos and implication for vector control

PLoS Neglected Tropical Diseases
Sébastien MarcombePaul T Brey


The yellow fever mosquito Aedes aegypti is the major vector of dengue, yellow fever, Zika, and Chikungunya viruses. Worldwide vector control is largely based on insecticide treatments but, unfortunately, vector control programs are facing operational challenges due to mosquitoes becoming resistant to commonly used insecticides. In Southeast Asia, resistance of Ae. aegypti to chemical insecticides has been documented in several countries but no data regarding insecticide resistance has been reported in Laos. To fill this gap, we assessed the insecticide resistance of 11 Ae. aegypti populations to larvicides and adulticides used in public health operations in the country. We also investigated the underlying molecular mechanisms associated with resistance, including target site mutations and detoxification enzymes putatively involved in metabolic resistance. Bioassays on adults and larvae collected in five provinces revealed various levels of resistance to organophosphates (malathion and temephos), organochlorine (DDT) and pyrethroids (permethrin and deltamethrin). Synergist bioassays showed a significant increased susceptibility of mosquitoes to insecticides after exposure to detoxification enzyme inhibitors. Biochemical assays c...Continue Reading


Dec 29, 1998·Emerging Infectious Diseases·William G Brogdon, J C McAllister
May 9, 2003·Nature·Mylene WeillMichel Raymond
Jul 10, 2004·Insect Biochemistry and Molecular Biology·J HemingwayHilary Ranson
Feb 26, 2008·Transactions of the Royal Society of Tropical Medicine and Hygiene·Basil D Brooke
Jul 1, 2009·Tropical Medicine & International Health : TM & IH·Julie ValléeJean-Paul Gonzalez
Aug 5, 2010·The American Journal of Tropical Medicine and Hygiene·Angela F HarrisHilary Ranson
Jan 1, 2008·Molecular Ecology Resources·François Rousset
Jun 4, 2011·Journal of Vector Ecology : Journal of the Society for Vector Ecology·Thipwara ChuaycharoensukTheeraphap Chareonviriyaphap
Aug 3, 2013·Western Pacific Surveillance and Response Journal : WPSAR·Chanthavy SoulaphyHannah C Lewis
Mar 22, 2014·PLoS Neglected Tropical Diseases·Rodolphe PoupardinHilary Ranson
Apr 25, 2017·PloS One·Sébastien MarcombePaul T Brey
Jul 21, 2017·PLoS Neglected Tropical Diseases·Catherine L MoyesDavid Weetman
Dec 29, 2017·PloS One·Somphavanh SomlorMarc Grandadam
Mar 6, 2018·Asia-Pacific Journal of Public Health·Sébastien BoyerJeffrey Hii