Distribution of mast cell subtypes in interstitial cystitis: implications for novel diagnostic and therapeutic strategies?

Journal of Clinical Pathology
Shabana T MalikBashir A Lwaleed

Abstract

To identify the presence and geographical distribution of mast cell (MC) subtypes: MCT (tryptase positive-chymase negative) and MCTC (tryptase positive-chymase positive) in bladder tissue. Bladder tissue was obtained from patients with painful bladder syndrome/interstitial cystitis (n=14) and normal histology from University Hospital Southampton tissue bank. Sequential tissue slices were immunohistochemically stained for MC subtypes using anti-MC tryptase (for MCT and MCTC) and anti-MC chymase (for MCTC). Stained sections were photographed, and positively stained MCs were quantified using ImageJ. Data were analysed using descriptive statistics and individual paired t-tests. There was a significant difference in the density of MCs between each layer of the disease bladder, with the greatest accumulation within the detrusor (p<0.001). There was a significant increase in MCTC subtype in the lamina (p=0.009) in painful bladder syndrome/interstitial cystitis. Our results suggest that mastocytosis is present within all layers of disease bladder, especially the muscle layer. The varying increase in MC subtypes in the lamina and mucosa may explain the variability in painful bladder syndrome/interstitial cystitis symptoms. A high influx...Continue Reading

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Citations

Oct 8, 2018·International Urogynecology Journal·Carolyn D Keagy
Apr 5, 2019·Neurourology and Urodynamics·Ji-Yeon HanMyung-Soo Choo
Sep 22, 2020·International Urogynecology Journal·Visha K TailorVik Khullar
Nov 13, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Zhijun LinMingqiang Li

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