Distribution of members of the PSD-95 family of MAGUK proteins at the synaptic region of inner and outer hair cells of the guinea pig cochlea

Synapse
C DaviesR S Petralia

Abstract

PDZ-domain containing proteins of the MAGUK (membrane-associated guanylate kinase) family target, anchor, and cluster receptors and channels to subcellular sites. Among the MAGUK proteins, the members of the PSD-95 family (MAGUKs: PSD-95, PSD-93, SAP-97, and SAP-102) target and anchor glutamate receptors to the synaptic terminals. Associations of glutamate receptors with MAGUKs have been described in the brain but not in the cochlea. In this study, RT-PCR, immunofluorescence microscopy, and immunoelectron microscopy were used to investigate the presence and distribution of MAGUK proteins in the organ of Corti. The presence of the mRNA for PSD-95, PSD-93, SAP-97, and SAP-102 in the organ of Corti was confirmed by RT-PCR. Immunocytochemistry using a "pan-MAGUK" antibody, which recognizes all four MAGUK proteins, and selective antibodies against these proteins revealed that all four MAGUKs are present within the base of inner hair cells while all except SAP-97 are found within the base of the outer hair cells. In addition, PSD-93 and PSD-95 are found in postsynaptic afferent terminals on inner hair cells, while postsynaptic afferent terminals on outer hair cells have PSD-93.

References

Apr 1, 1992·Neuroreport·R PujolM Eybalin
Mar 1, 1992·The Journal of Physiology·G D Housley, J F Ashmore
Mar 1, 1991·Hearing Research·K Ehrenberger, D Felix
Feb 1, 1990·The Journal of Physiology·C J Kros, A C Crawford
Aug 22, 1995·Proceedings. Biological Sciences·E GlowatzkiB Fakler
Apr 1, 1993·Hearing Research·P Wangemann, S Takeuchi
Dec 1, 1995·Progress in Neurobiology·J L Puel
Jun 16, 1997·The Journal of Cell Biology·T HassonD P Corey
Jun 1, 1997·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·C P PontingD J Blake
Jan 1, 1997·Audiology & Neuro-otology·A S NiedzielskiR J Wenthold
Jan 14, 1998·Neuron·E B Ziff
Feb 17, 1998·Neuroscience Letters·V N Kharazia, R J Weinberg
May 21, 1999·The European Journal of Neuroscience·P BassandM Khrestchatisky
Jul 27, 1999·The Journal of Physiology·J RuelJ L Puel
Aug 17, 1999·Current Opinion in Cell Biology·A S Fanning, J M Anderson
Oct 12, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·S D DimitratosP J Bryant
Nov 24, 1999·The American Journal of Physiology·T van Den AbbeeleP T Huy
Jun 22, 2000·Proceedings of the National Academy of Sciences of the United States of America·M DuanB Canlon
Jul 14, 2000·Acta Oto-laryngologica·L NordangM Anniko

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Citations

Jan 11, 2007·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·E KalayH Kremer
Jun 15, 2006·The Journal of Membrane Biology·R NouvianT Moser
Mar 12, 2009·Journal of the Association for Research in Otolaryngology : JARO·Joe C Adams
Sep 2, 2010·Purinergic Signalling·Narinobu Harada
Aug 20, 2004·Proceedings of the National Academy of Sciences of the United States of America·James A KapplerA J Hudspeth
Sep 24, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Inga U SchapitzMatthias Kneussel
Apr 2, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Chan-Ying ZhengRobert J Wenthold
May 27, 2014·Hearing Research·Taro FujikawaBechara Kachar
Nov 28, 2002·The Journal of Comparative Neurology·Patricia LangerAlfons Rüsch
Feb 11, 2014·Hearing Research·Wei-Ming Yu, Lisa V Goodrich
Apr 2, 2002·Nature Genetics·Seth G N Grant
Dec 29, 2018·Synapse·Thomas M CoateMansa Gurjar
Sep 22, 2006·Human Molecular Genetics·Hannie KremerRonald Roepman
Jun 15, 2007·The Journal of Biological Chemistry·Tonghui XuEbenezer N Yamoah
Jul 15, 2011·American Journal of Physiology. Cell Physiology·Jean DefournyBrigitte Malgrange
Feb 7, 2014·The Journal of Experimental Biology·Dwayne D SimmonsRichard A Baird

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