Abstract
[3H]N-acetylgalactosamine injected into the cell body of R2, the giant cholinergic neuron in the abdominal ganglion, is rapidly incorporated into membrane glycoprotein and glycolipid. Incorporation, which is localized to the injected cells, occurs at a constant rate for approximately 15 h. By that time, 83% of the labeled macromolecules are associated with membranes. Quantitative electron microscopic radioautography of the cell body shows that labeling of membranous organelles is selective: the Golgi apparatus, endoplasmic reticulum, and lucent and compound vesicles are labeled, while the nucleus, end-stage lysosomes, and mitochondria are not. SDS-polyacrylamide gel electrophoresis of the total membranes from more than 40 R2s examined individually resolves reproducibly 5 major labeled glycoprotein components. In order to determine which of these area associated with vesicles, we isolated a labeled vesicle fraction from R2 using a combination of differential centrifugation and filtration on a column of glass beads with 200 nm pores. This fraction was consistently enriched in [3H]glycoproteins I (180,000 Daltons) and V (90,000 Daltons) relative to those fractions containing larger organelles. These experiments suggest that differ...Continue Reading
References
Aug 29, 1975·Brain Research·R T AmbronJ H Schwartz
Jul 1, 1976·The Journal of Physiology·D J GoldbergJ H Schwartz
Jan 1, 1977·Neuroscience·E Holtzman
Jan 5, 1979·Science·A A SherbanyJ H Schwartz
Jan 1, 1979·Annual Review of Neuroscience·J H Schwartz
Jun 11, 1976·Brain Research·A NagyV P Whittaker
Jan 9, 1979·Biochemistry·S L HamiltonA Karlin
Dec 1, 1975·The Journal of Cell Biology·G Blobel, B Dobberstein
Feb 1, 1977·Brain Research·R T Ambron, S N Treistman
Aug 1, 1977·Journal of Neurochemistry·P M SalvaterraH R Mahler
Aug 13, 1976·Brain Research·E B ThompsonE R Kandel
Nov 1, 1976·The Journal of Cell Biology·P KellyG L Nicolson
Aug 1, 1976·The Journal of Cell Biology·J E GoldmanJ H Schwartz
Jun 1, 1974·The Journal of Cell Biology·R T AmbronJ H Schwartz
Jan 1, 1974·Annual Review of Biochemistry·J L Dahl, L E Hokin
Jul 1, 1974·The Journal of Cell Biology·C W Cotman, D Taylor
Aug 29, 1973·Nature: New Biology·A MatusM C Raff
Dec 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·M EisenstadtJ H Schwartz
Nov 1, 1972·Analytical Biochemistry·L H KirkegaardR M Bock
Jun 1, 1974·The Journal of Cell Biology·R T AmbronJ H Schwartz
Jul 16, 1974·Biochemistry·T L RosenberryE Bock
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey
Aug 15, 1970·Nature·U K Laemmli
Apr 16, 1971·Science·R J Lasek, W J Dower
Apr 7, 1971·Journal of Chromatography·C W HiattJ M Galimore
Apr 1, 1969·The Journal of Cell Biology·M M SalpeterE E Salpeter
Oct 1, 1980·Physiological Reviews·B Grafstein, D S Forman
Feb 23, 1981·Brain Research·R T AmbronJ H Schwartz
Citations
Feb 23, 1981·Brain Research·R T AmbronJ H Schwartz
Jul 29, 1982·Brain Research·H B Kistler, J H Schwartz
Apr 15, 1985·Brain Research·P F DrakeR J Lasek
May 13, 1982·Brain Research·R T Ambron